hFXYD6反义核酸对胆管癌细胞体外增殖和侵袭能力的影响
FXYD6;反义核酸;胆管癌;增殖;侵袭;荧光定量RT-PCR;免疫组化;流式细胞术,窦春青,周宁新,岳鑫,窦春青,通讯作者:,EffectofhumanantisenseFXYD6ontheproliferationandinv
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窦春青, 周宁新, 中国人民解放军总医院全军肝胆外科研究所 北京市 100853
岳鑫, 北京大学神经科学研究所 北京市 100083
窦春青, 博士, 主治医师, 主要从事胆道生理病理及胆管癌基因诊断治疗研究.
国家自然科学基金资助项目, No. 30471699
通讯作者: 周宁新, 100853, 北京市, 北京市解放军总医院全军肝胆外科研究所. zhounx301@163.com
收稿日期: 2006-12-25 接受日期: 2007-01-20
Effect of human antisense FXYD6 on the proliferation and invasion of human cholangiocarcinoma cells in vitro
Chun-Qing Dou, Xin Yue, Ning-Xin Zhou
Chun-Qing Dou, Ning-Xin Zhou,Institute of Hepatobiliary Surgery, General Hospital of Chinese PLA, Beijing 100853, China
Xin Yue,Neuroscience Research Institute, Peking University, Beijing 100083, China
Supported byNational Natural Science Foundation of China, No. 30471699
Correspondence to:Ning-Xin Zhou, Institute of Hepatobiliary Surgery, General Hospital of Chinese PLA, Beijing 100853, China. zhounx301@163.com
Received:2006-12-25 Accepted:2007-01-20
Abstract
AIM: To investigate the effect of human antisense FXYD6 (FXYD domain containing ion transport regulator 6) on the proliferation and invasion of human cholangiocarcinoma cell line QBC939 in vitro.
METHODS: Human cholangiocarcinoma cell line QBC939 was transfected with the plasmid expressing human antisense FXYD6. Meanwhile, the empty vector and non-transfection group were designed. The mRNA transcription level of hFXYD6 was assayed by real-time reverse transcriptase polymerase chain reaction (RT-PCR) with SYBR Green I, and the expression of hFXYD6 protein was detected by immunohistochemistry. MTT and the colony-forming assay were used to measure the ability of cell growth. The cell cycle distribution was analyzed by flow cytometry and Transwell chamber model was employed to test the ability of cell invasion in vitro.
RESULTS: In comparison with the cells transfected with empty vector or without transfection, QBC939 cells transfected with antisense hFXYD6 had a significant decrease in mRNA transcription and protein expression ......
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