葡萄糖神经酰胺合成酶基因在人胃癌细胞SGC-7901及SGC-7901/VCR的表达和意义
人胃癌细胞;葡萄糖神经酰胺合成酶;基因表达;多药耐药,张艳,贾丹辉,刘宗文,乐晓萍,张钦宪,通讯作者:,Expressionofglucosylceramidesynthasegeneandits
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张艳, 贾丹辉, 郑州大学基础医学院药理学教研室 河南省郑州市 450052
刘宗文, 河南省肿瘤病理重点实验室 河南省郑州市 450052
乐晓萍, 张钦宪, 郑州大学基础医学院组织胚胎学教研室 河南省郑州市 450052
通讯作者: 张钦宪, 450052, 郑州市大学路40号, 郑州大学基础医学院组织胚胎学教研室. qxz53@zzu.edu.cn
电话: 0371-66658153 传真: 0371-66658153
收稿日期: 2006-12-20 接受日期: 2007-01-10
Expression of glucosylceramide synthase gene and its significance in human gastric carcinoma SGC-7901 and SGC-7901/VCR cells
Yan Zhang, Zong-Wen Liu, Dan-Hui Jia, Xiao-Ping Le, Qin-Xian Zhang
Yan Zhang, Dan-Hui Jia,Department of Pharmacology, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450052, He’nan Province, China
Zong-Wen Liu,He’nan Key Laboratory for Tumor Pathology, Zhengzhou 450052, He’nan Province, China
Xiao-Ping Le, Qin-Xian Zhang,Department of Histology and Embryology, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450052, He’nan Province, China
Correspondence to:Dr. Qin-Xian Zhang, Department of Histology and Embryology, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450052, He’nan Province, China. qxz53@zzu.edu.cn
Received: 2006-12-20 Accepted:2007-01-10
Abstract
AIM: To investigate the expression of glucosylceramde synthase (GCS) gene and its significance in human gastric carcinoma SGC-7901 and SGC-7901/VCR cells.
METHODS: Human gastric carcinoma SGC-7901/VCR and SGC-7901 cells were cultured in vitro, and the total RNA was extracted by TRIzol method. MTT was adopted to evaluate the drug resistance of SGC-7901/VCR cells. Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the expression of GCS mRNA, and Western blotting and immunocytochemistry were performed to measure the expression of GCS protein.
RESULTS: The half inhibitory concentrations (IC50) for SGC-7901 and SGC-7901/VCR were 1.40 ± 0.06 and 86.20 ± 0 ......
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