胃癌MKN-28肿瘤细胞系SP细胞亚群初步分析
闁稿繑濞婇弫顓犳嫚瀹ュ繒绐�胃癌;MKN-28细胞;ABCG2;SP亚群;肿瘤干细胞,王宁,陈凛,卫勃,赵向阳,鲁文静,沈丽,通讯作者:,PrimaryanalysisofsidepopulationinhumanagastriccancercelllineMKN-28
![]() |
第1页 |
参见附件(828KB,4页)。
王宁, 陈凛, 卫勃, 赵向阳, 中国人民解放军总医院普通外科 北京市 100853
鲁文静, 沈丽, 北京大学干细胞研究中心 北京市 100083
国家自然科学基金资助项目, No. 30570522
通讯作者: 陈凛, 100853, 北京市复兴路28号, 中国人民解放军总医院普通外科. chenlinbj@vip.sina.com
电话: 010-66937846 传真: 010-68181689
收稿日期: 2007-01-11 接受日期: 2007-01-31
Primary analysis of side population in human a gastric cancer cell line MKN-28
Ning Wang, Lin Chen, Bo Wei, Xiang-Yang Zhao, Wen-Jing Lu, Li Shen
Ning Wang, Lin Chen, Bo Wei, Xiang-Yang Zhao,Department of General Surgery, General Hospital of Chinese PLA, Beijing 100853, China
Wen-Jing Lu, Li Shen,Center for Stem Cell Research, Peking University, Beijing 10083, China
Supported byNational Natural Science Foundation of China, No. 30570522
Correspondence to:Lin Chen, Department of General Surgery, General Hospital of Chinese PLA, 28 Fuxing Road, Beijing 100853, China. chenlinbj@vip.sina.com
Received:2007-01-11 Accepted: 2007-01-31
Abstract
AIM: To identify the side population (SP) of cancer stem-like cells in gastric cancer cell line MKN-28.
METHODS: Immunofluorescence assay was used to determine the expression of ABCG2, an indicator of stem cells, in gastric cancer cell line MKN-28. MKN-28 tumor cells suspension was stained with Hoechst33342 and propidiumiodide (PI) with the absence or presence of verapamil. Then the SP was analyzed by fluorescence-activated cell sorter.
RESULTS: A little amount of ABCG2 expression was found in MKN-28 cells, and the ABCG2-positive cells covered a percentage of 1.7%. SP cells accounted for 0.25% of the MKN-28 cells, which was decreased to 0.05% after the treatment of verapamil.
CONCLUSION: SP cancer stem-like cells exist in gastric cancer cell line MKN-28, and they can be decreased by verapamil.
Key Words: Gastric cancer; MKN-28 cells; ABCG2; Side population; Cancer stem cell
Wang N, Chen L, Wei B, Zhao XY, Lu WJ, Shen L ......
您现在查看是摘要介绍页,详见PDF附件(828KB,4页)。
闂傚嫭鍔掔花顒勫嫉瀹ュ懎顫ら柛锝冨妼鐢洭宕濆☉宕囩缂傚啯鍨归悵顖炴焾閵娿儱鐎诲ǎ鍥e墲娴煎懘宕i鍐繑闁绘牕宕缓鐐濮橆剚鍠呴柟瀛樼墬濠€浣圭▔閳ь剛鈧姘ㄨⅶ闁告帒妫涘▓鎴濃枖閵娿儱鏂€濞村吋鑹鹃幉鍐裁规担绛嬫綌闁靛棴鎷�
婵繐鎷� pdf闁稿繈鍔嶉弸锟� 闂傚洠鍋撻悷鏇嫹 5 缂佸鍨伴崹搴ㄦ晬閸繂甯抽悹鎰潐閺佺偤宕樺畝鈧▍銉ㄣ亹閺囩偞鍊�婵絽绻愰妵澶愬矗椤栨瑤绨板Λ鏉挎瑜帮拷10濞戞搩浜炶ⅶ闁告帪鎷�闁挎稑顦埀顒婃嫹
濞e洠鍓濇导鍛閸涱剛杩旈柛娆忓€介埀顒€鍠涚槐婵囩▔瀹ュ棛鈧垶骞嬮幇顏呭床濞达絾娲戠粻锝咁嚈妤︽鍞撮柕鍡曠劍鐢綊鎳¢幇顓炵仐闁圭ǹ娲ょ槐鈺呭Υ閸屾稒鐎紒鏃傚Х婢ф寮堕崘銊ф剑濞存粌楠哥敮顐︽媼濡炲墽绋婇柡澶婂暕濮瑰鏁嶅畝鍐仧闁诡喓鍔忛缁樼▔閻戞﹩鍔冮柡鍌氭矗缁楀鈧绮忛~锕傚绩鐠鸿櫣绉垮〒姘☉閵囧洨鈧娉涢崢銈囨嫻瑜版帗顫夐悹鍥︾串缁辨繄鎷犻悜钘変粡濞寸姾鍩栭崹銊╂偨娴e啰妯堥梺顐f皑閻擄繝骞嬮幋婊勭拨闁挎稑鏈崹婊勭椤掍焦鏆柛鎺嶅嵆閳ь剚姘ㄩ悡锟犲触鎼搭垳绀夊ù鍏兼皑閻濇盯宕¢崘鑼闁诡喓鍔庡▓鎴炴媴濠婂啯鎯傚ù鐘插濠€鎵磾閹寸姷褰查柛鎺斿█濞呭酣濡撮敓锟�
鐎甸偊鍠曟穱濠囧棘閸モ晝褰�
闁稿繗娅曢弫鐐烘儌閻愵剙顎�
閻犲洤瀚鎴﹀礄閻樻彃缍�
闁瑰吋绮庨崒銊╁即閺夋埈妯�
|