胃癌MKN-28肿瘤细胞系SP细胞亚群初步分析
胃癌;MKN-28细胞;ABCG2;SP亚群;肿瘤干细胞,王宁,陈凛,卫勃,赵向阳,鲁文静,沈丽,通讯作者:,PrimaryanalysisofsidepopulationinhumanagastriccancercelllineMKN-28
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王宁, 陈凛, 卫勃, 赵向阳, 中国人民解放军总医院普通外科 北京市 100853
鲁文静, 沈丽, 北京大学干细胞研究中心 北京市 100083
国家自然科学基金资助项目, No. 30570522
通讯作者: 陈凛, 100853, 北京市复兴路28号, 中国人民解放军总医院普通外科. chenlinbj@vip.sina.com
电话: 010-66937846 传真: 010-68181689
收稿日期: 2007-01-11 接受日期: 2007-01-31
Primary analysis of side population in human a gastric cancer cell line MKN-28
Ning Wang, Lin Chen, Bo Wei, Xiang-Yang Zhao, Wen-Jing Lu, Li Shen
Ning Wang, Lin Chen, Bo Wei, Xiang-Yang Zhao,Department of General Surgery, General Hospital of Chinese PLA, Beijing 100853, China
Wen-Jing Lu, Li Shen,Center for Stem Cell Research, Peking University, Beijing 10083, China
Supported byNational Natural Science Foundation of China, No. 30570522
Correspondence to:Lin Chen, Department of General Surgery, General Hospital of Chinese PLA, 28 Fuxing Road, Beijing 100853, China. chenlinbj@vip.sina.com
Received:2007-01-11 Accepted: 2007-01-31
Abstract
AIM: To identify the side population (SP) of cancer stem-like cells in gastric cancer cell line MKN-28.
METHODS: Immunofluorescence assay was used to determine the expression of ABCG2, an indicator of stem cells, in gastric cancer cell line MKN-28. MKN-28 tumor cells suspension was stained with Hoechst33342 and propidiumiodide (PI) with the absence or presence of verapamil. Then the SP was analyzed by fluorescence-activated cell sorter.
RESULTS: A little amount of ABCG2 expression was found in MKN-28 cells, and the ABCG2-positive cells covered a percentage of 1.7%. SP cells accounted for 0.25% of the MKN-28 cells, which was decreased to 0.05% after the treatment of verapamil.
CONCLUSION: SP cancer stem-like cells exist in gastric cancer cell line MKN-28, and they can be decreased by verapamil.
Key Words: Gastric cancer; MKN-28 cells; ABCG2; Side population; Cancer stem cell
Wang N, Chen L, Wei B, Zhao XY, Lu WJ, Shen L ......
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