环氧合酶-2和基质金属蛋白酶-2在大鼠非酒精性脂肪肝炎中的作用
环氧合酶-2;基质金属蛋白酶-2;逆转录聚合酶链反应;非酒精性脂肪性肝炎;大鼠,刘晓珺,沈滨,杜雅菊,刘晓珺,通讯作者:,Roleofcyclooxygenase-2andmatrixmetalloproteinase-
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刘晓珺, 哈尔滨医科大学附属第二医院急诊内科 黑龙江省哈尔滨市 150086
沈滨, 哈尔滨医科大学附属第二医院普外二科 黑龙江省哈尔滨市 150086
杜雅菊, 哈尔滨医科大学附属第二医院消化内科 黑龙江省哈尔滨市 150086
刘晓珺, 主治医师, 主要从事肝病的研究.
通讯作者: 杜雅菊, 150086, 黑龙江省哈尔滨市, 哈尔滨医科大学附属第二临床医院消化内科. duyaju@medmail.com
收稿日期: 2006-11-11 接受日期: 2006-12-18
Role of cyclooxygenase-2 and matrix metalloproteinase-2 in nonalcoholic steatohepatitis of rats
Xiao-Jun Liu, Bin Shen, Ya-Ju Du
Xiao-Jun Liu,Department of Emergency, the Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang Province, China
Bin Shen,Department of General Surgery, the Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang Province, China
Ya-Ju Du,Department of Gastroenterology, the Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang Province, China
Correspondence to:Ya-Ju Du, Department of Gastroenterology, the Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang Province, China. duyaju@medmail.com
Received:2006-11-11 Accepted: 2006-12-18
Abstract
AIM: To observe the roles of cyclooxygenase-2 (COX-2) and matrix metalloproteinase-2 (MMP-2) in the pathogenesis and development of rats with nonalcoholic steatohepatitis (NASH).
METHODS: Thirty Wistar rats were randomly divided into model group (n = 24) and control group (n = 6), treated with high-fat diet (100 g/L lard, 20 g/L cholesterol, 5 g/L sodium cholate and 875 g/L basal forage) and basal forage, respectively. Eight rats in the model group and 2 rats in the control group were killed randomly in the 12th, 16th and 24th week, respectively, and two pieces of liver tissues were obtained. One was fixed in 40 g/L formaldehyde solution and stained for the sake of histopathological observation and the other was stored in -80℃ refrigerator after being quickly frozen by liquid nitrogen in order to extract RNA and detect the relative amount of COX-2 mRNA and MMP-2 mRNA in liver tissues by reverse transcription-polymerase chain reaction (RT-PCR) ......
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