过氧化物酶体增殖剂激活受体-γ在白藜芦醇抑制胃癌SGC-7901细胞增殖中的作用
过氧化物酶体增殖剂激活受体-γ;细胞周期素D1;白藜芦醇;胃肿瘤,胡光胜,曹文涛,廖爱军,曾斌,廖丹,石巍,通讯作者:,Roleofperoxisomeproliferator-activatedreceptor-
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胡光胜, 曹文涛, 廖爱军, 曾斌, 廖丹, 石巍, 南华大学第一附属医院消化内科 湖南省衡阳市 421001
通讯作者: 胡光胜, 421001, 湖南省衡阳市, 南华大学第一附属医院消化内科. hugs7321@163.com
电话: 0734-8279064
收稿日期: 2006-09-08 接受日期: 2006-12-01
Role of peroxisome proliferator-activated receptor-γ in resveatrol-induced proliferation inhibition of gastric cancer cell line SGC-7901
Guang-Sheng Hu, Wen-Tao Cao, Ai-Jun Liao, Bin Zeng, Dan Liao, Wei Shi
Guang-Sheng Hu, Wen-Tao Cao, Ai-Jun Liao, Bin Zeng, Dan Liao, Wei Shi,Department of Gastroenterology, the First Affiliated Hospital of Nanhua University, Hengyang 421001, Hunan Province, China
Correspondence to: Guang-Sheng Hu, Department of Gastroenterology, the First Affiliated Hospital of Nanhua University, Hengyang 421001, Hunan Province, China. hugs7321@163.com
Received:2006-09-08 Accepted:2006-12-01
Abstract
AIM: To investigate the role of peroxisome proliferator-activated receptor-γ (PPAR-γ) in the inhibitory effects of resveratrol (Res) on gastric cancer cell SGC-7901 proliferation in vitro.
METHODS: After human gastric cancer cell SGC-7901 was cultured in vitro, MTT assay was used to detect the inhibitory effect of Res and GW9662 (specific blocker of PPAR-γ) on cell proliferation. Flow cytometry was used to detect cell cycle, and PPAR-γ, Cyclin D1 mRNA was measured quantitatively by reverse transcription-polymerase chain reaction (RT-PCR). The expression of PPAR-γ protein was measured quantitatively by Western blot, and the expression of Cyclin D1 protein in gastric cancer cells was detected by immunohistochemistry.
RESULTS: Res inhibited the proliferation of SGC-7901 cells in a dose- and time-dependent manner (P < 0.05), and flow cytometry analysis revealed that the cell cycle was blocked at G1-phase. RT-PCR and Western blot showed that both PPAR-γ mRNA and protein were expressed in SGC-7901 cells, and Res enhanced PPAR-γ mRNA expression. After SGC-7901 cells were treated by Res with the concentration of 25, 50, 75 and 100 μmol/L, the relative percentages of PPAR-γ mRNA expression were 122 ......
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