金雀异黄素对人胃腺癌细胞中Akt及其磷酸化蛋白表达的影响
金雀异黄素;胃癌;丝,苏氨酸蛋白激酶,付荣霞,杨艳梅,张春艳,付荣霞,通讯作者:,EffectofGenisteinontheexpressionofAktandphosphorylatedAktsinhumangastricca
 |
| 第1页 |
参见附件(891KB，5页)。
付荣霞, 天津农学院食品科学系生物工程教研室 天津市 300384
杨艳梅, 张春艳, 哈尔滨医科大学肿瘤研究所 黑龙江省哈尔滨市 150081
付荣霞, 医学博士, 讲师, 主要从事营养与生物技术的研究.
天津农学院科学研究发展基金资助项目, No. 20030002
通讯作者: 付荣霞, 300384, 天津市西青区津静公路22号, 天津农学院食品科学系生物工程教研室. furongxia@sina.com.cn
电话: 022-23782596 传真: 022-23782596
收稿日期: 2007-02-06 接受日期: 2007-03-07
Effect of Genistein on the expression of Akt and phosphorylated Akts in human gastric carcinoma cell line
Rong-Xia Fu, Yan-Mei Yang, Chun-Yan Zhang
Rong-Xia Fu,Biological Engineering Laboratory, Department of Food Science, Tianjin Agricultural University, Tianjin 300384, China
Yan-Mei Yang, Chun-Yan Zhang, Institute of Cancer Research, Harbin Medical University, Harbin 150081, Heilongjiang Province, China
Supported bythe Science Research and Developed Foundation of Tianjin Agriculture University, No. 20030002
Correspondence to:Rong-Xia Fu, Biological Engineering Laboratory, Department of Food Science, Tianjin Agricultural University, Tianjin 300384, China. furongxia@sina.com.cn
Received:2007-02-06 Accepted: 2007-03-07
Abstract
AIM: To investigate the effects of Genestein on the expression of Akt and its phosphorylated proteins (p-Akts) in human gastric carcinoma cell line SGC-7901.
METHODS: SGC-7901 cells were exposed to 0, 5, 10, 20, 40 and 80 μmol/L Genistein for 24 hours. The effect of Genistein on cell proliferation was measured by methyl thiazolyl tetrazolium (MTT) assay, and the apoptosis of SGC-7901 cells was evaluated by Hoechst 33342 staining. The expression of Akt and p-Akts (Ser473 and Thr308) were measured by Western blot.
RESULTS: The inhibitory rate was 6.85% ± 3.71%, 13.19% ± 1.90%, 20.94% ± 1.83%, 29.58% ± 1.19% and 41.75% ± 1.92%, respectively, as SGC-7901 cells were exposed to 5, 10, 20, 40 and 80 μmol/L Genistein for 24 hours. Typical morphological changes of apoptosis were induced by Genistein at the concentrations (20-80 μmol/L) ......
您现在查看是摘要介绍页，详见PDF附件(891KB，5页)。