P2X受体对豚鼠胃窦环行肌自发性收缩运动的影响
α,b-MeATP;P2X嘌呤受体;胃窦环行肌,黄旭,张扬,高玲,赵鹏,韩燕飞,许文燮,黄旭,通讯作者:,EffectofP2Xreceptoronthespontaneouscontractionofgastricantralcircular
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黄旭, 张扬, 高玲, 赵鹏, 韩燕飞, 许文燮, 上海交通大学医学院生理学教研室 上海市 200240
黄旭, 2004年上海交通大学医学院硕士生, 主要从事消化生理学的研究.
国家自然科学基金资助项目, No. 30360031
通讯作者: 许文燮, 200240, 上海市东川路800号, 上海交通大学医学院文选医学楼328室. wenxiexu@sjtu.edu.cn
电话: 021-34205639
收稿日期: 2006-10-30 接受日期: 2006-12-18
Effect of P2X receptor on the spontaneous contraction of gastric antral circular muscle in guinea pigs
Xu Huang, Yang Zhang, Ling Gao, Peng Zhao, Yan-Fei Han, Wen-Xie Xu
Xu Huang, Yang Zhang, Ling Gao, Peng Zhao, Yan-Fei Han, Wen-Xie Xu,Department of Physiology, Medical College of Shanghai Jiao Tong University, Shanghai 200240, China
Supported byNational Natural Science Foundation of China, No. 30360031
Correspondence to:Professor Wen-Xie Xu, Shanghai Jiao Tong University, Room 328, Wenxuan Medical Building, 800 Dongchuan Road, Shanghai, 200240, China. wenxiexu@sjtu.edu.cn
Received:2006-10-30 Accepted:2006-12-18
Abstract
AIM: To investigate the effect of alpha, beta-methylene ATP (α,b-MeATP) on gastric smooth muscle motility and its ionic channel mechanism in guinea pigs.
METHODS: EWG/B guinea pig was used in this study. Gastric antral smooth muscle strips (10 × 1.5 mm) were prepared and then fixed in the organ bath system (37℃) which were perfused with sodium bicarbonate buffer solution and aerated (950 mL/L O2, 50 mL/L CO2). The spontaneous contraction of gastric antral circular muscle was recorded by SMUP-E bio-electric signal-processing system. The single gastric myocyte was dispersed with type Ⅱ collagenase, and the ionic current and membrane potential were recorded using whole-cell patch clamp technique in freshly dispersed smooth muscle cells, including calcium-activated potassium current, delayed rectifier potassium current, and voltage-dependent calcium current.
RESULTS: α,b-MeATP, a P2X receptor agonist, significantly inhibited the spontaneous contraction of gastric antral circular muscle in a dose-dependent manner, and the contraction extent were decreased from 100% to 90% ± 2%, 81% ± 4%, 68% ± 4%, 59% ± 7% and 29% ± 4% when α,b-MeATP was used at the concentrations of 5, 10, 20, 40 and 100 μmol/L (P < 0 ......
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