α-干扰素对活化的肝星状细胞早期凋亡的影响
肝星状细胞;α-干扰素;肝纤维化;早期凋亡,车雷,马力,吴丹,李智伟,通讯作者:,Effectsofinterferon-αontheearlyapoptosisofactivatedhepaticstellatecells,LeiChe,LiM
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车雷, 马力, 吴丹, 李智伟, 中国医科大学附属第二医院感染科 辽宁省沈阳市 110004
通讯作者: 李智伟, 110004, 辽宁省沈阳市和平区三好街36号, 中国医科大学附属第二医院感染科. sy901015@sina.com
电话: 024-83956860
收稿日期: 2007-02-02 接受日期: 2007-03-06
Effects of interferon-α on the early apoptosis of activated hepatic stellate cells
Lei Che, Li Ma, Dan Wu, Zhi-Wei Li
Lei Che, Li Ma, Dan Wu, Zhi-Wei Li,Department of Infectious Diseases, China Medical University, Shenyang 110004, Liaoning Province, China
Correspondence to:Zhi-Wei Li, Department of Infectious Diseases, China Medical University, Shenyang 110004, Liaoning Province, China. sy901015@sina.com
Received:2007-02-02 Accepted:2007-03-06
Abstract
AIM: To investigate the effects of interferon-α on the early apoptosis of activated hepatic stellate cells (HSCs), and to further explore the anti-fibrosis mechanism of interferon-α (IFN-α).
METHODS: Rat HSCs were cultured in vitro, and then treated with different concentrations of IFN-α for 24 hours. The viable cell count was measured by MTT assay, and the early apoptosis of HSCs was detected by flow cytometry using AnnexinV/PI-staining.
RESULTS: MTT assay showed a decrease of HSCs after IFN-α treatment, and the absorbency was significantly different from that of the control cells when 1 × 107 and 1 × 108 U/L IFN-α was used (t = 2.82, 5.44, both P < 0.05). Flow cytometry demonstrated that the early apoptosis rate of HSCs was increased with the elevation of IFN-α concentration, and the apoptosis rate was markedly different from that of the control cells when IFN-α was adopted at the concentrations of 1 × 107 and 1 × 108 U/L (t= 4.31, 5.07, both P < 0.05).
CONCLUSION: IFN-α can induce the early apoptosis of HSCs, which is probably one of the anti-fibrosis mechanism of interferon-α.
Key Words: Hepatic stellate cell; Interferon-α; Liver fibrosis; Early apoptosis ......
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