卡巴胆碱对肠上皮细胞氧化损伤的保护作用
卡巴胆碱;上皮细胞;大鼠;MTT法,邹晓防,胡森,吕艺,盛志勇,孙娜,石新慧,李泽峰,通讯作者:,Protectiveeffectsofcarbacholonintestinalepithelialcellsagainstoxidativeinjury
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邹晓防, 胡森, 吕艺, 盛志勇 中国人民解放军总医院第一附属医院烧伤研究所休克与多器官功能障碍实验室 北京市 100037
孙娜, 石新慧, 李泽峰, 中国人民解放军总医院第一附属医院检验科 北京市 100037
军队"十一五"医药卫生专项课题项目, No. 06Z055
通讯作者: 胡森, 100037, 北京市, 北京市中国人民解放军总医院第一附属医院烧伤研究所休克与多器官功能障碍实验室. hs82080@yahoo.com.cn
电话: 010-66867397 传真: 010-68989139
收稿日期: 2007-02-25 接受日期: 2007-03-31
Protective effects of carbachol on intestinal epithelial cells against oxidative injury
Xiao-Fang Zou, Sen Hu, Yi Lv, Na Sun, Xin-Hui Shi, Ze-Feng Li, Zhi-Yong Sheng
Xiao-Fang Zou, Sen Hu, Yi Lv, Zhi-Yong Sheng,Burn Research Institute, the First Affiliated Hospital of Chinese PLA General Hospital, Beijing 100037, China
Na Sun, Xin-Hui Shi, Ze-Fen Li,Laboratory of Shock and Multiple Organ Dysfunction, Department of Laboratory, the First Affiliated Hospital of Chinese PLA General Hospital, Beijing 100037, China
Supported bythe Special Foundation of Military Medical Project during the 11th Five-Year Period, No. 06Z055
Correspondence to:Sen Hu, Laboratory of Shock and Multiple Organ Dysfunction, Burn Research Institute, the First Affiliated Hospital of Chinese PLA General Hospital, Beijing 100037 Beijing, China. hs82080@yahoo.com.cn
Received:2007-02-25 Accepted:2007-03-31
Abstract
AIM: To study the effects of carbachol on the H2O2-oxidative injury of intestinal epithelial cells (IEC).
METHODS: IEC were cultured with the presence of H2O2in vitro to induce the oxidative injury. IEC were divided into 3 groups: control group, H2O2 (2.5 mmol/L) group and carbachol (100 μmol/L) group. The viability of IEC was measured by MTT assay. The level of lactate dehydrogenase (LDH) in culture media and the malondialdehyde (MDA) content in IEC were measured.
RESULTS: In compared with those in control group, LDH level (7 ......
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