STAT5信号转导通路及其靶基因产物与结直肠癌恶性潜能的关系
结直肠癌;信号转导;转录信号转导子与激活子;CyclinD1;Caspase-3;Westernblot,欧云菘,马向涛,余力伟,通讯作者:,ConstitutiveactivationofSTAT5pathwayandoverex
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欧云菘, 马向涛, 余力伟, 北京海淀医院外科 北京市 100080
国家自然科学基金资助项目, No. 30271269
通讯作者: 马向涛, 100080, 北京市海淀区中关村大街29号, 北京海淀医院外科. xiangtao_ma@pku.org.cn
电话: 010-62639764 传真: 010-62583027
收稿日期: 2007-01-08 接受日期: 2007-02-09
Constitutive activation of STAT5 pathway and overexpression of target gene products correlate with malignant potential in human colorectal carcinoma
Yun-Song Ou, Xiang-Tao Ma, Li-Wei Yu
Yun-Song Ou, Xiang-Tao Ma, Li-Wei Yu,Department of Surgery, Beijing Haidian Hospital, Beijing 100080, China
Supported by National Natural Science Foundation of China, No. 30271269
Correspondence to:Dr. Xiang-Tao Ma, Department of Surgery, Beijing Haidian Hospital, 29 Zhongguancun Avenue, Haidian District, Beijing 100080, China. xiangtao_ma@pku.org.cn
Received:2007-01-08 Accepted:2007-02-09
Abstract
AIM: To investigate the expression of signal transducer and activator of transcription 5 (STAT5) and target gene products including Cyclin D1 and Caspase-3 in human colorectal carcinoma (CRC), and to explore the mechanism in the tumorigenesis of CRC.
METHODS: Primary colorectal cancer and adjacent normal mucosal specimens were obtained from 60 patients undergoing CRC resection at Beijing Haidian Hospital from December 2003 to December 2005. Western blot analysis was used to measure the expression of STAT5, p-STAT5, Cyclin D1, and Caspase-3 in the cancerous and adjacent normal tissues.
RESULTS: The levels of p-STAT5, Cyclin D1, and Caspase-3 protein were increased in the cancer tissues as compared with those in the normal ones (P = 0.028, 0.035, 0.046). Over-expression of p-STAT5 was correlated with TNM staging (P = 0.026), and Caspase-3 expression was also associated with TNM staging in CRC (P = 0.041). Caspase-3 was in a positive linear correlation with p-STAT5 in tumor (r = 0.412, P < 0.05).
CONCLUSION: STAT5 signaling pathway may play an important role in the tumorigenesis of CRC, and detection of STAT5 and its target gene products may predict the malignant potential of CRC.
Key Words: Colorectal neoplasm; Signal transduction; Signal transducer and activator of transcription 5; Cyclin D1; Caspase-3; Western blot
Ou YS, Ma XT, Yu LW. Constitutive activation of STAT5 pathway and overexpression of target gene products correlate with malignant potential in human colorectal carcinoma. Shijie Huaren Xiaohua Zazhi 2007;15(11):1306-1309
摘要
目的: 分析STAT5及其靶基因产物Cyclin D1与Caspase-3在结直肠癌组织中的表达情况, 探讨STAT5及其靶基因产物在结直肠癌发病机制中的作用.
方法: 收集北京海淀医院外科2003-12/ 2005-12经手术切除的结直肠癌标本60例, 应用Western blot检测60例结直肠癌组织、正常黏膜中STAT5及其靶基因产物Cyclin D1与Caspase-3表达.
结果: p-STAT5, Cyclin D1及Caspase-3表达水平在结直肠癌组织中明显高于正常黏膜(P = 0.028, 0.035, 0.046); p-STAT5表达水平与分期相关(P = 0.026); Caspase-3与分期相关(P = 0.041). p-STAT5与Caspase-3在结直肠癌组织中表达情况呈线性相关(r = 0.412, P<0.05).
结论: STAT5信号转导通路可能在结直肠癌发生过程中起重要作用, 检测结直肠癌中STAT5及其靶基因产物的表达可以反映肿瘤的恶性潜能 ......
欧云菘, 马向涛, 余力伟, 北京海淀医院外科 北京市 100080
国家自然科学基金资助项目, No. 30271269
通讯作者: 马向涛, 100080, 北京市海淀区中关村大街29号, 北京海淀医院外科. xiangtao_ma@pku.org.cn
电话: 010-62639764 传真: 010-62583027
收稿日期: 2007-01-08 接受日期: 2007-02-09
Constitutive activation of STAT5 pathway and overexpression of target gene products correlate with malignant potential in human colorectal carcinoma
Yun-Song Ou, Xiang-Tao Ma, Li-Wei Yu
Yun-Song Ou, Xiang-Tao Ma, Li-Wei Yu,Department of Surgery, Beijing Haidian Hospital, Beijing 100080, China
Supported by National Natural Science Foundation of China, No. 30271269
Correspondence to:Dr. Xiang-Tao Ma, Department of Surgery, Beijing Haidian Hospital, 29 Zhongguancun Avenue, Haidian District, Beijing 100080, China. xiangtao_ma@pku.org.cn
Received:2007-01-08 Accepted:2007-02-09
Abstract
AIM: To investigate the expression of signal transducer and activator of transcription 5 (STAT5) and target gene products including Cyclin D1 and Caspase-3 in human colorectal carcinoma (CRC), and to explore the mechanism in the tumorigenesis of CRC.
METHODS: Primary colorectal cancer and adjacent normal mucosal specimens were obtained from 60 patients undergoing CRC resection at Beijing Haidian Hospital from December 2003 to December 2005. Western blot analysis was used to measure the expression of STAT5, p-STAT5, Cyclin D1, and Caspase-3 in the cancerous and adjacent normal tissues.
RESULTS: The levels of p-STAT5, Cyclin D1, and Caspase-3 protein were increased in the cancer tissues as compared with those in the normal ones (P = 0.028, 0.035, 0.046). Over-expression of p-STAT5 was correlated with TNM staging (P = 0.026), and Caspase-3 expression was also associated with TNM staging in CRC (P = 0.041). Caspase-3 was in a positive linear correlation with p-STAT5 in tumor (r = 0.412, P < 0.05).
CONCLUSION: STAT5 signaling pathway may play an important role in the tumorigenesis of CRC, and detection of STAT5 and its target gene products may predict the malignant potential of CRC.
Key Words: Colorectal neoplasm; Signal transduction; Signal transducer and activator of transcription 5; Cyclin D1; Caspase-3; Western blot
Ou YS, Ma XT, Yu LW. Constitutive activation of STAT5 pathway and overexpression of target gene products correlate with malignant potential in human colorectal carcinoma. Shijie Huaren Xiaohua Zazhi 2007;15(11):1306-1309
摘要
目的: 分析STAT5及其靶基因产物Cyclin D1与Caspase-3在结直肠癌组织中的表达情况, 探讨STAT5及其靶基因产物在结直肠癌发病机制中的作用.
方法: 收集北京海淀医院外科2003-12/ 2005-12经手术切除的结直肠癌标本60例, 应用Western blot检测60例结直肠癌组织、正常黏膜中STAT5及其靶基因产物Cyclin D1与Caspase-3表达.
结果: p-STAT5, Cyclin D1及Caspase-3表达水平在结直肠癌组织中明显高于正常黏膜(P = 0.028, 0.035, 0.046); p-STAT5表达水平与分期相关(P = 0.026); Caspase-3与分期相关(P = 0.041). p-STAT5与Caspase-3在结直肠癌组织中表达情况呈线性相关(r = 0.412, P<0.05).
结论: STAT5信号转导通路可能在结直肠癌发生过程中起重要作用, 检测结直肠癌中STAT5及其靶基因产物的表达可以反映肿瘤的恶性潜能 ......
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