幽门螺杆菌BALB/c小鼠适应性菌株的驯化及模型建立
幽门螺杆菌;动物模型;BALB,c小鼠;感染;适应性菌株,郭刚,刘开云,解庆华,张卫军,邹全明,郭刚,通讯作者:,EstablishmentofaBALB,cmousemodelinfectedsteadilywithHpylorithr
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郭刚, 刘开云, 解庆华, 张卫军, 邹全明, 第三军医大学临床微生物学及免疫学教研室 重庆市 400038
郭刚, 副教授, 博士, 主要从事病原细菌诊防方面的研究.
国家自然科学基金资助项目, No. 30400019
通讯作者: 邹全明, 400038, 重庆市, 第三军医大学临床微生物及免疫学教研室. guog@mail.tmmu.com.cn
电话: 023-68752315 传真: 023-68752315
收稿日期: 2007-01-23 接受日期: 2007-02-13
Establishment of a BALB/c mouse model infected steadily with H pylori through serial passages in vivo
Gang Guo, Kai-Yun Liu, Qing-Hua Xie, Wei-Jun Zhang, Quan-Ming Zou
Gang Guo, Kai-Yun Liu, Qing-Hua Xie, Wei-Jun Zhang, Quan-Ming Zou,Department of Clinnical Microbiology and Immunology, the Third Military Medical University, Chongqing 400038, China
Supported byNational Natural Science Foundation of China, No. 30400019
Correspondence to:Dr. Quan-Ming Zou, Department of Clinical Microbiology and Immunology, the Third Military Medical University, Chongqing 400038, China. guog@mail.tmmu.com.cn
Received:2007-01-23 Accepted:2007-02-13
Abstract
AIM: To increase the colonization ability of H pylori and establish a BALB/c mouse model infected steadily with H pylori.
METHODS: Mongolian gerbils-adaptive strain GS10 of H pylori were inoculated into BALB/c mice and acclimated through serial passagesin vivo for procuring an adaptive colonization of H pylori strain. After being infected for 4 weeks, all the animals were treated for microbiological examination, including culturing, smearing, urease test and polymerase chain reaction (PCR). The infectious rate of each group was calculated till the rate rose to 80%. Then, the gastric mucosal tissues were collected from the infected mice for quantitative analysis of H pylori and histological examination.
RESULTS: The proportion of BALB/c mice infected by H pylori increased gradually from about 11.1% at the first infection to 80% after 6 passages in vivo. At the 4th week after BALB/c mice were infected with the adaptive strain BS8 (strains after 8 passages), the colonization density in the gastric antrum, body and fundus of the infected mice, expressed by the common logarithm of colony forming unit per gram of tissue, was 5 ......
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