厦门市乙型肝炎患者病毒基因型的分析
乙型肝炎病毒;基因型;基因重组;巢式PCR法;特异性引物,董菁,任建林,王琳,卢雅丕,林逊汀,林振和,林辉,廉亚美,董菁,通讯作者:,PreliminarystudyonhepatitisBvirusgenotypesinXiamencity,Jing
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董菁, 任建林, 王琳, 卢雅丕, 林逊汀, 林振和, 林辉, 廉亚美, 厦门大学医学院附属中山医院消化内科 福建省厦门市 361004
董菁, 医学博士, 副教授, 副主任医师, 主要从事肝癌发病机制方面的研究.
厦门市首批重大疾病科研攻关项目, No. WKZ0501
厦门市卫生局医学科研立项项目, No. WSK0506
厦门大学引进人才科研启动基金, No. Z03109
通讯作者: 董菁, 361004, 福建省厦门市, 厦门大学医学院附属中山医院消化内科. dj1550@xmu.edu.cn
电话: 0592-2292017
收稿日期: 2006-04-06 接受日期: 2006-04-29
Preliminary study on hepatitis B virus genotypes in Xiamen city
Jing Dong, Jian-Lin Ren, Lin Wang, Ya-Pi Lu, Xun-Ting Lin, Zhen-He Lin, Hui Lin, Ya-Mei Lian
Jing Dong, Jian-Lin Ren, Lin Wang, Ya-Pi Lu, Xun-Ting Lin, Zhen-He Lin, Hui Lin, Ya-Mei Lian,Department of Gastroenterology, Zhongshan Hospital Affiliated to Medical College of Xiamen University, Xiamen 361004, Fujian Province, China
Supported byXiamen Municipal Foundation for Major Diseases, No. WKZ0501, the Medical Research Granted Project of Xiamen Health Bureau, No, WSK0506, and the Scientific Research Launch Fund for Introduction of Talents into Xiamen University, No. Z03109
Correspondence to:Dr. Jing Dong, Department of Gastroenterology, Zhongshan Hospital Affiliated to Medical College of Xiamen University, Xiamen 361004, Fujian Province, China. dj1550@xmu.edu.cn
Received: 2006-04-06 Accepted:2006-04-29
Abstract
AIM: To investigate the genotypes of hepatitis B virus (HBV) in Xiamen city by nested polymerase chain reaction (PCR) with multiplex pairs of genotype-specific primers.
METHODS: A total of 250 HBV-infected patients were included in this study. The serum samples were collected and the serum HBV DNA was used as templates. Ten outer and inner primers were designed on the basis of nucleotide sequences in the regions of Pre-S1 and S genes, of which 8 genotype-specific inner primers were divided into 2 groups: A and B. Genotype A, B, C or D, E, F of HBV were amplified, respectively. The genotypes of the second-round PCR products were identified using agarose gel electrophoresis (30 g/L) ......
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