吉西他滨对人胰腺癌Patu-8988细胞株APE/Ref-1的诱导作用
胰腺肿瘤;无嘌呤嘧啶核酸内切酶;吉西他滨;逆转录-聚合酶链反应;免疫印迹,熊光苏,吴叔明,徐晓晶,周鋆,朱红音,李恩灵,莫剑忠,通讯作者:,ExpressionofAPE,Ref-1inhumanpancreaticcanc
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熊光苏, 吴叔明, 徐晓晶, 周鋆, 朱红音, 李恩灵, 莫剑忠, 上海交通大学医学院附属仁济医院消化内科, 上海市消化疾病研究所 上海市 200001
上海市领先学科建设项目, No. Y0205
上海交通大学医学院博士点基金项目, No. BXJ0617
通讯作者: 吴叔明, 200001, 上海市山东中路145号, 上海交通大学医学院附属仁济医院, 上海市消化疾病研究所.
wusm2002@hotmail.com
电话: 021-53882027
收稿日期: 2007-01-24 接受日期: 2007-02-08
Expression of APE/Ref-1 in human pancreatic cancer cell line Patu-8988 induced by gemcitabine
Guang-Su Xiong, Shu-Ming Wu, Xiao-Jing Xu, Jun Zhou, Hong-Yin Zhu, En-Ling Li, Jian-Zhong Mo
Guang-Su Xiong, Shu-Ming Wu, Xiao-Jing Xu, Jun Zhou, Hong-Yin Zhu, En-Ling Li, Jian-Zhong Mo,Department of Gastroenterology, Renji Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 20001, China
Supported byShanghai Leading Academic Discipline Project, No. Y0205, and the Foundation of the Authorization Spot for Doctor's Degree of Shanghai Jiao Tong University School of Medicine, No. BXJ0617
Correspondence to:Shu-Ming Wu, Department of Gastroenterology, Renji Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, 145 Shandong Middle Road, Shanghai 20001, China. wusm2002@hotmail.com
Received:2007-01-24 Accepted:2007-02-08
Abstract
AIM: To investigate the changes of APE/Ref-1 expression in human pancreatic cancer cells after induction of gemcitabine.
METHODS: Human pancreatic cancer cell line Patu-8988 was incubated with 0, 10, 20, 40 and 60 μmol/L gemcitabine for 24 hours. The mRNA and protein levels of APE/Ref-1 were determined by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot, respectively.
RESULTS: Twenty-four hours after treatment with gemcitabine, the expression of APE/Ref-1 were elevated significantly both at mRNA and protein level, and was positively correlated with the concentrations of gemcitabine (RT-PCR: r = 0.645, P = 0.012; Western blot:r = 0.598, P = 0.020).
CONCLUSION: Up-regulated APE/Ref-1 expression may be an adaptive response contributing to the overall chemoresistance in pancreatic carcinomas, which implies the potential theraputic effect targeting on APE/Ref-1 ......
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