应用竞争性荧光定量聚合酶链反应检测乙型肝炎病毒DNA
乙型肝炎病毒;竞争性荧光定量聚合酶链反应;假阴性,刘蓉,李明远,张发强,夏增亮,闫乃红,王玲,卢亦路,夏庆杰,陈清英,卢军,夏军,通讯作者:,DetectionofhepatitisBvirusDNAbycompetitivefluo
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刘蓉, 李明远, 四川大学华西基础医学与法医学院微生物学教研室 四川省成都市 610041
张发强, 夏增亮, 闫乃红, 王玲, 卢亦路, 夏庆杰, 四川大学华西分子遗传实验室 四川省成都市 610041
陈清英, 卢军, 夏军, 重庆市涪陵区生殖健康保健中心 重庆市 40800
国家自然科学基金资助课题, No. 39880025
通讯作者: 夏庆杰, 610041, 四川省成都市高新区科园四路1号, 四川大学华西医院分子遗传实验室. xiaqj2005@126.com
电话: 028-81812855 传真: 028-85164005
收稿日期: 2007-01-15 接受日期: 2007-02-08
Detection of hepatitis B virus DNA by competitive fluorescence quantitative polymerase chain reaction
Rong Liu, Ming-Yuan Li, Fa-Qiang Zhang, Zeng-Liang Xia, Nai-Hong Yan, Ling Wang, Yi-Lu Lu, Qing-Jie Xia, Qing-Ying Chen, Jun Lu, Jun Xia
Rong Liu, Ming-Yuan Li,Department of Microbiology, West China College of Basic Medical Science and Forensic Medicine, Sichuan University, Chengdu 610041, Sichuan Province, China
Fa-Qiang Zhang, Zeng-Liang Xia, Nai-Hong Yan, Ling Wang, Yi-Lu Lu, Qing-Jie Xia,Laboratory of Molecular Genetics, West China Hospital of Sichuan University, Chengdu 610041, Sichuan Province, China
Qing-Ying Chen, Jun Lu, Jun Xia,Reproductive Health Care Center of Fuling District, Chongqing 40800, China
Supported byNational Natural Science Foundation of China, No. 39880025
Correspondence to:Qing-Jie Xia, Laboratory of Molecular Genetics, West China Hospital of Sichuan University, 1 Keyuan Fourth Road, High-Tech Development Zone, Chengdu 610041, Sichuan Province, China. xiaqj2005@126.com
Received:2007-01-15 Accepted:2007-02-08
Abstract
AIM: To set up a competitive fluorescence quantitative polymerase chain reaction (CFQ-PCR) for decreasing the false negative ratio in HBV DNA detection.
METHODS: According to HBV adr subtype gene, a pair of HBV-specific primer as well as a specific TaqMan probe was synthesized ......
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