反义RNA对胃癌细胞MT1-MMP基因表达和侵袭性的抑制作用
膜型1-基质金属蛋白酶;反义RNA;人胃癌细胞;逆转录-聚合酶链反应;MTT法;明胶酶谱;体外侵袭实验,胡楠,周晓武,付汉江,邢瑞云,郑晓飞,胡楠,孔维,胡楠,通讯作者:,Inhibitoryeffectofantisen
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胡楠, 周晓武, 中国人民解放军空军总医院普通外科 北京市 100036
付汉江, 邢瑞云, 郑晓飞, 军事医学科学院放射医学研究所 北京市 100850
胡楠, 孔维, 吉林大学生命科学学院 吉林省长春市 130023
胡楠, 在读硕士研究生, 主要进行基因治疗的研究.
空军“十一五”重点科研课题资助项目, No. KJZ20013022
通讯作者: 周晓武, 100036, 北京市海淀区阜成路30号, 中国人民解放军空军总医院普通外科. drzhouxiaowu@sina.com
电话: 010-68410099-6301
收稿日期: 2007-01-18 接受日期: 2007-02-13
Inhibitory effect of antisense RNA on the gene expression of membrane-type 1 matrix metalloproteinase and invasiveness of human gastric carcinoma cells
Nan Hu, Xiao-Wu Zhou, Han-Jiang Fu, Rui-Yun Xing, Wei Kong, Xiao-Fei Zheng
Nan Hu, Xiao-Wu Zhou,Department of General Surgery, General Hospital of Chinese PLA Air Force, Beijing 100036, China
Han-Jiang Fu, Rui-Yun Xing, Xiao-Fei Zheng,Insititute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China
Nan Hu, Wei Kong,College of Life Science, Jilin University, Changchun 130023, Jilin Province, China
Supported bythe Key Scientific Research Foundation of Chinese PLA Air Force during the 11th Five-Year Plan Period, No. KJZ20013022
Correspondence to:Xiao-Wu Zhou, Department of General Surgery, General Hospital of Chinese PLA Air Force, Beijing 100036, China. drzhouxiaowu@sina.com
Received:2007-01-18 Accepted: 2007-02-13
Abstract
AIM: To investigate the influence on the gene expression of membrane-type 1 matrix metalloproteinase (MT1-MMP) and invasiveness of human gastric carcinoma cell line BGC823 by antisense RNA.
METHODS: The eukaryotic expression vector carrying MT1-MMP antisense RNA was constructed with recombinant technology and then transfected into human gastric cancer cell line BGC823. The changes of MT1-MMP mRNA expression, cell proliferation, activation of gelatinase A and cell invasion ability were examined by reverse transcription-polymerase chain reaction (RT-PCR), MTT assay, zymography and Transwell invasion assay, respectively ......
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