乙型肝炎肝纤维化组织双向电泳技术的优化
乙型肝炎;肝纤维化组织;双向凝胶电泳,罗新华,张权,程明亮,杨勤,通讯作者:,Optimizationoftwo-dimensionalgelelectrophoresistechniqueforliverfibrosistissuefrompatie
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罗新华, 张权, 程明亮, 贵阳医学院附属医院感染科 贵州省贵阳市 550004
杨勤, 贵阳医学院病理生理学教研室 贵州省贵阳市 550004
贵州省优秀青年科技人才基金资助, No. (2005)0518
贵州省优秀科技教育人才省长专项基金资助项目, No. (2005)223
贵州省卫生厅科技基金资助项目, No. (2006)046
通讯作者: 程明亮, 550004, 贵州省贵阳市北京路4号, 贵阳医学院附属医院感染科. chengml@21cn.com
电话: 0851-6813394 传真: 0851-6813394
收稿日期: 2007-01-31 接受日期: 2007-03-06
Optimization of two-dimensional gel electrophoresis technique for liver fibrosis tissue from patients with hepatitis B
Xin-Hua Luo, Qin Yang, Quan Zhang, Ming-Liang Cheng
Xin-Hua Luo, Quan Zhang, Ming-Liang Cheng,Department of Infectious Diseases, the Affiliated Hospital of Guiyang Medical College, Guiyang 550004, Guizhou Province, China
Qin Yang,Department of Pathophysiology, Guiyang Medical College, Guiyang 550004, Guizhou Province, China
Supported bythe Foundation for the Excellent Young Talents of Science and Technology of Guizhou Province, No. (2005)0518; the Nomarch Special Foundation for the Excellent Science and Technology Talents of Guizhou Province, No.(2005)223; and the Science and Technology Foundation of Health Department of Guizhou Province, No. (2006)046
Correspondence to:Dr. Ming-Liang Cheng, Department of Infectious Diseases, the Affiliated Hospital of Guiyang Medical College, 4 Beijing Road, Guiyang 550004, Guizhou Province, China. chengml@21cn.com
Received:2007-01-31 Accepted:2007-03-06
Abstract
AIM: To establish and optimize the technique of two-dimensional gel electrophoresis (2-DE) for studying the proteomics of liver fibrosis tissue from patients with hepatitis B.
METHODS: Liver fibrosis tissues were obtained by surgical method, and the total proteins were extracted. Immobilized pH gradient isoelectric two-dimensional gel electrophoresis was used to separate the total protein. After coomassie brilliant blue staining, 2-DE maps were analyzed with ImageMaster 2D Platinum Software ......
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