胃癌相关基因GCRG213正反向转染和RNA干扰对裸鼠胃癌种植瘤的影响
胃癌相关基因GCRG213;腺相关病毒;小干扰RNA;基因转染;胃癌种植瘤,徐世平,吴本俨,王孟薇,高利利,伍银桥,王卫华,尤纬缔,通讯作者:,Effectofsenseoranti-senseGCRG213fragmenta
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徐世平, 吴本俨, 王孟薇, 高利利, 伍银桥, 王卫华, 尤纬缔, 中国人民解放军总医院南楼消化科 北京市 100853
国家自然科学基金资助项目, No.30370635
通讯作者: 吴本俨, 100853, 北京市海淀区复兴路28号, 中国人民解放军总医院南楼消化科. benyanwu@vip.sina.com
电话: 010-66939443
收稿日期: 2007-04-04 接受日期: 2007-04-21
Effect of sense or anti-sense GCRG213 fragment and GCRG213 RNA interference on transplanted gastric carcinoma in vivo
Shi-Ping Xu, Ben-Yan Wu, Meng-Wei Wang, Li-Li Gao, Yin-Qiao Wu, Wei-Hua Wang, Wei-Di You
Shi-Ping Xu, Ben-Yan Wu, Meng-Wei Wang, Li-Li Gao, Yin-Qiao Wu, Wei-Hua Wang, Wei-Di You, Department of Gastroenterology, South Building, General Hospital of Chinese PLA, Beijing 100853, China
Supported by National Natural Science Foundation of China, No. 30370635
Correspondence to: Dr. Ben-Yan Wu, Department of Gastroenterology, South Building, General Hospital of Chinese PLA, 28 Fuxing Road, Haidian District, Beijing 100853, China. benyanwu@vip.sina.com
Received: 2007-04-04 Accepted: 2007-04-21
Abstract
AIM: To investigate the influence of sense or anti-sense GCRG213 fragment and GCRG213 RNA interference (RNAi) on transplanted gastric cancer in vivo.
METHODS: Gastric carcinoma cell line MKN45 at logarithmic growth phase was subcutaneously inoculated in 20 athymic mice. After tumor formation, the adeno-associated virus (AAV) containing sense or anti-sense GCRG213 fragment and GCRG213 RNA interference fragment was injected into tubercles. Meanwhile, empty and normal saline control groups were designed. All the mice were killed 2 weeks after injection. Tumors were weighted and the expression of GCRG213 mRNA was detected by semi-quantitative reverse transcription-polymerase chain reaction.
RESULTS: Three weeks after inoculation of MKN45 cells, small tumor tubercles came into formation. The weights of tumor tissues were 5.12 ± 1.02, 1.22 ± 0.46, 0.81 ± 0.37, 3.13 ± 0.69 and 3.45 ± 0.87 g in sense, anti-sense, RNAi, empty and normal saline group, and the mRNA expression of GCRG213 were 0 ......
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