真核表达载体pcDNA3-CTLA4Ig转染小鼠对移植胰岛功能的影响
胰岛移植;排斥反应;共刺激信号;B7,CD28,赵国华,孙宏治,钟鑫平,赵宁,程颖,张佳林,刘永锋,张金祥,许国岩,赵国华,通讯作者:,PreventiveeffectofpcDNA3-CTLA4Igplasmidtrans
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赵国华, 孙宏治, 钟鑫平, 赵宁, 程颖, 张佳林, 刘永锋, 中国医科大学附属第一医院器官移植科 辽宁省沈阳市 110001
张金祥, 许国岩, 解放军65735部队医院内科 辽宁省丹东市 118002
赵国华, 2006年中国医科大学博士毕业, 主治医师, 主要从事消化系统疾病的研究.
辽宁省自然科学基金资助项目, No. 20042096
通讯作者: 赵国华, 110001, 辽宁省沈阳市和平区南京北街155号, 中国医科大学附属第一医院器官移植科.
zgh1975070707@163.com
电话: 024-82001796
收稿日期: 2007-01-30 接受日期: 2007-02-13
Preventive effect of pcDNA3-CTLA4Ig plasmid transfection on islet allograft rejection in mice
Guo-Hua Zhao, Hong-Zhi Sun, Xin-Ping Zhong, Jin-Xiang Zhang, Guo-Yan Xu, Ning Zhao, Ying Cheng, Jia-Lin Zhang, Yong-Feng Liu
Guo-Hua Zhao, Hong-Zhi Sun, Xin-Ping Zhong, Ning Zhao, Ying Cheng, Jia-Lin Zhang, Yong-Feng Liu, Organ Transplant Unit, the First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China
Jin-Xiang Zhang, Guo-Yan Xu, Department of Gastroenterology, the 65735th Hospital of Chinese PLA, Dandong 118002, Liaoning Province, China
Supported by the Natural Science Foundation of Liaoning Province, No. 20042096
Correspondence to: Guo-Hua Zhao, Organ Transplant Unit, the First Affiliated Hospital of China Medical University, 155 Nanjing North Street, Heping Disrict, Shenyang 110001, Liaoning Province, China. zgh1975070707@163.com
Received: 2007-01-30 Accepted: 2007-02-13
Abstract
AIM: To study the effect of pcDNA3-CTLA4Ig plasmid on islet allograft rejection in mice.
METHODS: C57BL/6 mice were randomly divided into control group, blank group and experiment group (transfected with pcDNA3-CTLA4Ig plasmid). The level of serum CTLA4Ig was detected by Western blot on day 5 after transfection. Blood glucose was examined after operation every other day. Immunohistochemical staining was used to determine the expression of insulin. 3H-thymidine incorporation was performed to detect the ability of T lymphocyte increment and the levels of CD4+ and CD8+ T lymphocytes were detected by flow cytometry on day 7 after transplantation ......
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