胎盘滋养层细胞的感染和凋亡与HBV的宫内传播机制
乙型肝炎病毒;人绒毛膜滋养层细胞系JEG-3;细胞免疫荧光;免疫组化;荧光定量聚合酶链式反应;原位缺口末端标记技术;宫内传播,白菡,张琳,冯国和,石理兰,窦晓光,赵桂珍,何丽霞,白菡,博士,讲师,通讯作者:,Relationshipbet
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白菡, 张琳, 冯国和, 石理兰, 窦晓光, 赵桂珍, 中国医科大学附属盛京医院感染科 辽宁省沈阳市 110004
何丽霞, 中国医科大学附属盛京医院妇产科 辽宁省沈阳市 110004
白菡, 博士, 讲师, 主要从事乙型肝炎病毒的母婴传播机制研究.
辽宁省自然科学基金资助项目, No.9910500706
通讯作者: 窦晓光, 110004, 辽宁省沈阳市, 中国医科大学附属盛京医院感染科. baihan910@163.com
电话: 024-83956961 传真: 024-83955089
收稿日期: 2007-03-27 接受日期: 2007-04-13
Relationship between the infection and apoptosis of placental trophoblastic cells and the mechanism of hepatitis B virus intrauterine transmission
Han Bai, Lin Zhang, Li-Xia He, Guo-He Feng, Li-Lan Shi, Xiao-Guang Dou, Gui-Zhen Zhao
Han Bai, Lin Zhang, Guo-He Feng, Li-Lan Shi, Xiao-Guang Dou, Gui-zhen Zhao,Department of Infectious Diseases, Shengjing Hospital Affiliated to China Medical University, Shenyang 110004, Liaoning Province, China
Li-Xia He,Department of Gynaecology and Obstetrics, Shengjing Hospital Affiliated to China Medical University, Shenyang 110004, Liaoning Province, China
Supported bythe Natural Science Foundation of Liaoning Province, No. 9910500706
Correspondence to:Dr. Xiao-Guang Dou, Department of Infectious Diseases, Shengjing Hospital Affiliated to China Medical University, Shenyang 110004, Liaoning Province, China. baihan910@163.com
Received:2007-03-27 Accepted:2007-04-13
Abstract
AIM: To study the mechanism of hepatitis B virus (HBV) intrauterine transmission by infecting the cultured human trophoblastic cells in vitro.
METHODS: Primary cultured trophoblastic cells from human first-trimester placenta and serial subcultivation of JEG-3 cell line were investigated. The primary cultured and subcultured cells were cultured in the HBV-infected serum for 8 to 48 hours. The appearance of the cells and intercellular conjunctions were observed under inverted microscope. The protein expression of HBsAg and HBcAg were detected by cell immunofluorescence and immunohistochemical staining ......
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