二甲双胍对高脂饲养大鼠肝脏脂肪变的干预作用
非酒精性脂肪性肝病;胰岛素抗药性;二甲双胍;聚合酶链式反应;酶联免疫测定,陈世清,黎华,刘杞,通讯作者:,Effectsofmetforminonhepaticsteatosisinducedinratsfedahighfatdiet,Shi-Q
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陈世清, 黎华, 郧阳医学院附属太和医院 湖北省十堰市 442000
刘杞, 重庆医科大学病毒性肝炎研究所 教育部感染性疾病分子生物学重点实验室 重庆市 400010
通讯作者: 刘杞, 400010, 重庆市渝中区临江路72号, 重庆医科大学病毒性肝炎研究所. liuqiz@hotmail.com
电话: 0719-8452796
收稿日期: 2007-03-06 接受日期: 2007-03-23
Effects of metformin on hepatic steatosis induced in rats fed a high fat diet
Shi-Qing Chen, Hua Li, Qi Liu
Shi-Qing Chen, Hua Li, Taihe Hospital, Yunyang Medical College, Shiyan, 442000, Hubei Province, China
Qi Liu, Institute for Viral Hepatitis, Key Laboratory for Molecular Biology of Infectious Diseases of Education Minstry, Chongqing Medical University, Chongqing 400010, China
Correspondence to: Qi Liu, Institute for Viral Hepatitis, Chongqing Medical University, 72 Linjiang Road, Yuzhong District, Chongqing 400010, China. liuqiz@hotmail.com
Received: 2007-03-06 Accepted: 2007-03-23
Abstract
AIM: To investigate the role and mechanism of insulin resistance in nonalcoholic fatty liver disease (NAFLD), and to evaluate the intervening effects of metformin (Met) on rats with hepatic steatosis induced by feeding a high fat diet.
METHODS: Twenty-one male Wistar rats were randomly divided into three equal groups. Rats in standard chow group (SD) were fed normal food, those in the high fat diet group (HF) had a high fat diet, and rats in the HF-Met group were fed a high fat diet but gavaged with Met for 8 weeks. Rats were sacrificed at the end of 8 weeks to weigh epididymal fat and calculate the liver index. Levels of ALT, AST, insulin, TG, TC, FFA, SOD, and MDA were detected by biochemistry, and fasting insulin by radioimmunoassay. mRNA and protein expression of hepatic TNF-α were detected by reverse-transcriptase polymerase chain reaction (RT-PCR) and Enzyme linked immunosorbent assay (ELISA), respectively. Insulin sensitivity was measured with Glucose Infusion Rate (GIR) by the euglycermic hyperinsulinemia clamp technique ......
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