自创微需氧罐优选幽门螺杆菌培养与保存条件
微需氧罐;幽门螺杆菌;培养;保存,周华,徐帆,李平,楚更五,通讯作者:,OptimizationofcultivationandpreservationofHelicobacterpyloriusingself-mademicroaerophilicp
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周华, 昆明医学院第一附属医院放疗科 云南省昆明市 650032
徐帆, 成都军区昆明总医院药剂科 云南省昆明市 650032
李平, 楚更五, 云南中医学院基础部 云南省昆明市 650200
王根春, 成都军区昆明总医院微生物室 云南省昆明市 650032
云南省自然科学基金资助项目, No. 2003C0089M
通讯作者: 李平, 650200, 云南省昆明市关上双桥路201号, 云南中医学院基础部. kmliping08@126.com
电话: 0871-7150987 传真: 0871-7150987
收稿日期: 2007-01-25 接受日期: 2007-03-06
Optimization of cultivation and preservation of Helicobacter pylori using self-made microaerophilic pot Hua Zhou, Fan Xu, Ping Li, Geng-Wu Chu, Gen-Chun Wang
Hua Zhou, Department of Radiotherapy, the First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan Province, China
Fan Xu, Department of Pharmacy, Kunming General Hospital of Chengdu Military Region, Kunming 650032, Yunnan Province, China
Ping Li, Geng-Wu Chu, Basic Department, Yunnan College of Chinese Traditional Medicine, Kunming 650032, Yunnan Province, China
Gen-Chun Wang, Department of Microbiology, Kunming General Hospital of Chengdu Military Region, Kunming 650032, Yunnan Province, China
Supported by The Natural Science Foundation of Yunnan Province, China, No. 2003C0089M
Correspondence to: Ping Li, Basic Department, Yunnan College of Chinese Traditional Medicine, 201 Shuangqiao Road, Guanshang, Kunming 650032, Yunnan Province, China. kmliping08@126.com
Received: 2007-01-25 Accepted: 2007-03-06
Abstract
AIM: To optimize the operation and condition of cultivation and preservation for Helicobacter pylori.
METHODS: Self-made microaerophilic pot was used to optimize the cultivation condition for H pylori with orthogonal designing, and the anabiosis of H pylori was observed and compared in two preservative fluids of different concentration under -20℃ ......
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