PARP-1抑制剂PJ34对人肝癌细胞株HepG2的抑制作用
聚腺苷酸二磷酸核糖转移酶-1;PJ34;肝癌;HepG2细胞;增殖抑制;流式细胞仪,周欣,黄志勇,陈孝平,黄胜辉,通讯作者:,Suppressiveeffectofpoly(ADP-ribose)polymerase-1inhibito
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周欣, 黄志勇, 陈孝平, 黄胜辉, 华中科技大学同济医学院附属同济医院肝脏外科中心 湖北省武汉市 430030
教育部新世纪优秀人才支持计划项目资助, No.NCET-04-0701
通讯作者: 黄志勇, 430030, 湖北省武汉市解放大道1095号, 华中科技大学同济医学院附属同济医院肝脏外科中心. zyhuang@medmail.com.cn
电话: 027-83663871 传真: 027-83663400
收稿日期: 2007-02-26 接受日期: 2007-03-31
Suppressive effect of poly (ADP-ribose) polymerase-1 inhibitor PJ34 on human hepatoma cell line HepG2
Xin Zhou, Zhi-Yong Huang, Xiao-Ping Chen, Sheng-Hui Huang
Xin Zhou, Zhi-Yong Huang, Xiao-Ping Chen, Sheng-Hui Huang, Hepatic Surgical Center, the Affiliated Tongji Hospital of Tongji Medical College, Huazhong Universty of Science and Technology, Wuhan 430030, Hubei Province, China
Supported by New Century Excellent Talent Program of Education Ministry, No. NCET-04-0701
Correspondence to: Zhi-Yong Huang, Hepatic Surgical Center, the Affiliated Tongji Hospital of Tongji Medical College, Huazhong Universty of Science and Technology, 1095 Jiefang Road, Wuhan 430030, Hubei Province, China. zyhuang@medmail.com.cn
Received: 2007-02-26 Accepted: 2007-03-31
Abstract
AIM: To evaluate the inhibitory effect and its mechanism of poly (ADP-ribose) polymerase-1 inhibitor, PJ34, on the growth of human hepatoma cell line, and investigate whether PJ34 can further enhance the suppression of γ-rays on HepG2 cell growth.
METHODS: Cell proliferation test was conducted to observe the effects of various concentrations of PJ34 or PJ34 in combination with γ-rays on the growth of HepG2 cells. Flow cytometry was used to examine the apoptosis rate in the PJ34-treated HepG2 cells.
RESULTS: PJ34 significantly suppressed the growth of HepG2 cells (t = 15.175, P < 0.01). The suppressive effects were enhanced with the increase of PJ34 concentration. 1Gy γ-rays also significantly inhibited HepG2 cell growth, but there was no significant difference in cell growth between the group with γ-rays or PJ34 alone and the group with combination (t = -1 ......
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