结直肠癌组织中ECM1基因水平的测定及临床意义
细胞外基质蛋白1;结直肠癌;实时定量聚合酶链反应,侯彦强,彭亮,娄加陶,周琳,孔宪涛,仲人前,倪健,通讯作者:,DetectionandclinicalsignificanceofECM1geneexpressionincolorectalcan
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侯彦强, 彭亮, 上海市第一人民医院松江分院检验科 上海市201600
娄加陶, 周琳, 孔宪涛, 仲人前, 中国人民解放军第二军医大学长征医院实验诊断科全军临床免疫中心 上海市 200003
倪健, 上海富纯中南生物技术有限公司 上海市 201702
国家自然科学基金项目, No. 30080027
上海市基础研究重大项目, No. 02JC14005
上海市松江区科学技术攻关项目, No. 06KG27
通讯作者: 仲人前, 200003, 上海市, 中国人民解放军第二军医大学长征医院实验诊断科, 全军临床免疫中心. rqzhong@yahoo.com
收稿日期: 2007-02-17 接受日期: 2007-03-31
Detection and clinical significance of ECM1 gene expression in colorectal cancer tissue
Yan-Qiang Hou, Jia-Tao Lou, Liang Peng, Lin Zhou, Jian Ni, Xian-Tao Kong, Ren-Qian Zhong
Yan-Qiang Hou, Liang Peng, Department of Clinical Laboratory, Songjiang Branch Hospital, the First People’s Hospital of Shanghai City, Shanghai 201600, China
Jia-Tao Lou, Lin Zhou, Xian-Tao Kong, Ren-Qian Zhong, Department of Laboratory Diagnosis, Changzheng Hospital of the Second Military Medical University, Clinical Immunology Center of Chinese PLA, Shanghai 200003, China
Jian Ni, Shanghai Fuchun Zhongnan Biotech Company, Shanghai 201702, China
Supported by: National Natural Science Foundation of China, No. 30080027, Shanghai Basic Research Major Project, No. 02JC14005 and the Key Item from Science and Technology Department of Shongjiang District in Shanghai, No. 06KG27
Correspondence to: Ren-Qian Zhong, Department of Laboratory Diagnosis, Changzheng Hospital of the Second Military Medical University, Clinical Immunology Center of ChinesePLA, Shanghai 200003, China. rqzhong@yahoo.com
Received: 2007-02-17 Accepted: 2007-03-31
Abstract
AIM: To investigate the expression and significance of the ECM1 gene in colorectal cancer tissue.
METHODS: Based on TaqMan-MGB methodology, real time quantitative polymerase chain reaction (RQ-PCR) was used to detect the mRNA levels of ECM1 in tissue from normal controls (n = 46), colorectal adenoma (n = 18), colorectal cancer without lymphatic metastasis (n = 25) and colorectal cancer with lymphatic metastasis (n = 21) ......
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