结肠癌耐药细胞株LoVo/5-FU的建立及其差异表达基因的筛选
结肠癌;耐药相关基因;5-氟尿嘧啶;LoVo,5-FU细胞株;MTT法;基因芯片;逆转录-聚合酶链反应,盛雅萍,郑磊贞,陈强,郭伟剑,李建芳,刘炳亚,罗建明,盛雅萍,通讯作者:,Establishmentandselection
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盛雅萍, 郑磊贞, 陈强, 郭伟剑, 上海交通大学医学院附属新华医院肿瘤科 上海市 200092
李建芳, 刘炳亚, 上海交通大学医学院附属瑞金医院消化外科研究所 上海市 200025
罗建明, 上海复旦大学附属肿瘤医院中心实验室 上海市200032
盛雅萍, 2002年南京医科大学本科毕业, 2004年至今就读于上海交通大学医学院, 肿瘤学硕士研究生.
通讯作者: 郭伟剑, 200092, 上海交通大学医学院附属新华医院肿瘤科. guoweijian1@sohu.com
电话: 021-65010796
收稿日期: 2007-02-06 接受日期: 2007-03-01
Establishment and selection of genes expressing differently in a human colon carcinoma LoVo/5-FU cell line
Ya-Ping Sheng, Jian-Fang Li, Bing-Ya Liu, Jian-Ming Luo, Lei-Zhen Zheng, Qiang Chen, Wei-Jian Guo
Ya-Ping Sheng, Lei-Zhen Zheng, Qiang Chen, Wei-Jian Guo, Department of Oncology, Xinhua Hospital Affiliated to Medical College of Shanghai Jiao Tong University, Shanghai, 200092, China
Jian-Fang Li, Bing-Ya Liu, Research Institute of Surgical Gastroenterology, Ruijing Hospital Affiliated to Medical College of Shanghai Jiao Tong University, Shanghai, 200025, China
Jian-Ming Luo, Department of Center Laboratory, Tumor Hospital of Fudan University, Shanghai, 200032, China
Correspondence to: Wei-Jian Guo, Department of Oncology, Xinhua Hospital of Medical College of Jiaotong University, Shanghai 200092, China. guoweijian1@sohu.com
Received: 2007-02-06 Accepted: 2007-03-01
Abstract
AIM: To establish a Lovo/5-FU of human drug-resistance colon carcinoma cell line, and to screen possible drug resistance-associated genes.
METHODS: LoVo/5-FU of a human drug-resistance colon carcinoma cell line was induced by continuously exposing human colon carcinoma cells to gradually increasing concentrations of 5-FU. The growth law was observed and the growth curve protracted. The drug resistance of LoVo/5-FU was measured by MTT assay and the drug resistant index (RI) was calculated. Genes expressing differently between LoVo/5-FU and its parent cell line Lovo were screened using a gene chip, and several selected associated drug resistance genes were confirmed by reverse transcription-polymerase chain reaction (RT-PCR) ......
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