SV40T抗原胃壁细胞特异性表达载体的构建与鉴定
SV40病毒;T抗原;真核表达;载体构建,陈辉,乐晓平,金辉,马慧洁,张钦宪,侯艺芳,陈辉,通讯作者:,ConstructionandidentificationofaspecificexpressionvectorforSV40la
 |
| 第1页 |
参见附件(873KB，5页)。
陈辉, 郑州大学基础医学院细胞生物学与医学遗传学教研室; 河南省分子医学重点实验室 河南省郑州市 450052
乐晓平, 金辉, 马慧洁, 张钦宪, 郑州大学基础医学院组织胚胎学教研室 河南省郑州市 450052
侯艺芳, 郑州大学第一附属医院妇产科 河南省郑州市 450052
陈辉, 1990年武汉大学生物系本科毕业, 在读博士研究生, 副教授, 主要从事遗传病诊断与分子遗传学研究.
河南省重大科技攻关计划资助项目, No. 0623031400
通讯作者: 张钦宪, 450052, 郑州市大学路40号, 郑州大学基础医学院组织胚胎学教研室. qxz53@zzu.edu.cn
电话: 0371-66658153 传真: 0371-66658153
收稿日期: 2007-01-11 接受日期: 2007-03-23
Construction and identification of a specific expression vector for SV40 large T antigen in gastric parietal cell
Hui Chen, Yi-Fang Hou, Xiao-Ping Le, Hui Jin, Hui-Jie Ma, Qin-Xian Zhang
Hui Chen,Department of Cell Biology and Medical Genetics, College of Basic Medicine Sciences of Zhengzhou University; He’nan Provincial Key Laboratory of Molecular Medicine, Zhengzhou 450052, He’nan Province, China
Xiao-Ping Le, Hui Jin, Hui-Jie Ma, Qin-Xian Zhang,Department of Histology and Embryology, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450052, He’nan Province, China
Yi-Fang Hou,Department of Obstetrics and Gynecology, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, He’nan Province, China
Supported by:He’nan Provincial Key Technologies R & D Program, No. 0623031400
Correspondence to:Dr. Qin-Xian Zhang, Department of Histology and Embryology, College of Basic Medical Sciences of Zhengzhou University, Zhengzhou 450052, He’nan Province, China. qxz53@zzu.edu.cn
Received:2007-01-11 Accepted:2007-03-23
Abstract
AIM: To construct and identify a eukaryotic specific expression vector of SV40 large T antigen in gastric parietal cell.
METHODS: Genome DNA was extracted from liver cells of Kunming mice by a phenol-chloroform method. The H+/K+ ATPase β subunit promoter gene was amplified by polymerase chain reaction (PCR) and the product was named as HK ......
您现在查看是摘要介绍页，详见PDF附件(873KB，5页)。