缺氧时三氧化二砷对人肝癌细胞株BEL-7402生长及凋亡的影响及机制
三氧化二砷;缺氧;凋亡;缺氧诱导因子1α;耐药基因1,刘晓川,刘铁夫,刘晓川,通讯作者:,Effectsandmechanismofarsenictrioxideongrowthandapoptosisofthehuman
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刘晓川, 刘铁夫, 哈尔滨医科大学附属第四临床医院消化内科 黑龙江省哈尔滨市 150001
刘晓川, 哈尔滨医科大学消化内科博士, 主要从事消化系统肿瘤的研究.
黑龙江省卫生厅基金资助项目, No. 2006-236
通讯作者: 刘铁夫, 150001, 黑龙江省哈尔滨市南岗区颐园街37号, 哈尔滨医科大学附属第四临床医学院消化内科.
tfliu@mail.csoft.com.cn
电话: 0451-82576696
收稿日期: 2007-03-01 接受日期: 2007-03-31
Effects and mechanism of arsenic trioxide on growth and apoptosis of the human liver cancer cell line BEL-7402 during hypoxia
Xiao-Chuan Liu, Tie-Fu Liu
Xiao-Chuan Liu, Tie-Fu Liu,Department of Gastroenterology, Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China
Supported by:Foundation from Sanitation Office, Heilongjiang Province, No. 2006-236
Correspondence to:Dr. Tie-Fu Liu, Department of Gastroenterology, Fourth Affiliated Hospital of Harbin Medical University, No. 37 Yiyuan Street, Nangang District, Harbin 150001, Heilongjiang Province, China. tfliu@mail.csoft.com.cn
Received:2007-03-01 Accepted:2007-03-31
Abstract
AIM: To investigate the effects of arsenic trioxide (As2O3) on apoptosis and migration of the human liver cancer cell BEL-7402 under normal and hypoxic conditions.
METHODS: A hypoxic environment was induced by CoCl2. The effect of As2O3 was determined by MTT assay. Electron microscopy, laser scanning confocal microscopy and flow cytometry (FCM) were used to study apoptosis of the human liver cancer cell BEL-7402. Expression of the multidrug resistance 1 (mdr1) and Hypoxia-inducible-factor-1α (HIF-1α) genes was detected by reverse transcriptase-polymerase chain reaction.
RESULTS: Different concentrations of As2O3 can inhibit growth and induce apoptosis of the human liver cancer cell BEL-7402 during hypoxia. After being induced by 4.0 μmol/L As2O3 for 48 hours in hypoxia, the BEL-7402 cell had morphological features of apoptosis as observed by electron and laser scanning confocal microscopies ......
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