巨噬细胞金属弹力酶对小鼠原位结肠癌生长及COX-2表达的影响
巨噬细胞金属弹力酶;基因克隆;结肠癌;环氧合酶-2;动物模型,石海,许建明,胡乃中,梅俏,鲍峻峻,石海,通讯作者:,Effectsofmousemacrophagemetalloelastaseongrowthandcycloox
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石海, 许建明, 胡乃中, 梅俏, 鲍峻峻, 安徽医科大学第一附属医院消化内科 安徽省合肥市 230022
石海, 副教授, 医学博士, 主要从事胃肠道肿瘤的基础与临床研究.
通讯作者: 许建明, 230022, 安徽省合肥市, 安徽医科大学第一附属医院消化内科. xjm1017@yahoo.com.cn
电话: 0551-2922039
收稿日期: 2007-03-13 接受日期: 2007-04-13
Effects of mouse macrophage metalloelastase on growth and cyclooxygenase-2 expression of murine orthotopic colon cancer
Hai Shi, Jian-Ming Xu, Nai-Zhong Hu, Qiao Mei, Jun-Jun Bao
Hai Shi, Jian-Ming Xu, Nai-Zhong Hu, Qiao Mei, Jun-Jun Bao,Department of Gastroenterology, First Affiliated Hospital of Anhui Medical University, Hefei 230022, Anhui Province, China
Correspondence to:Jian-Ming Xu, Department of Gastroenterology, First Affiliated Hospital of Anhui Medical University, Hefei 230022, Anhui Province, China. xjm1017@yahoo.com.cn
Received:2007-03-13 Accepted: 2007-04-13
Abstract
AIM: To determine correlations between macrophage metalloelastase (MME), microvessel angiogenesis and cyclooxygenase-2 (COX-2) expression in the angiogenesis of colon cancer.
METHODS: A cDNA fragment coding for domainsⅠand Ⅱ of MME was transfected into MME deficient murine CT-26 colon cancer cells. The enzymatic activity of recombinant MME was confirmed by cleavage of native substrate in vitro. An orthotopic implantation model was established using MME-transfected and control cells. Tumor samples were subjected to Western blot to detect expressions of MME and COX-2. Microvessel counting was used to assess angiogenesis of murine colon tumors.
RESULTS: Primary tumors with volumes and microvessel formation carrying pcDNA3.1-transfected and nontransfected cells were larger than that in the group implanted with MME-transfected (1151.07 ± 35.91 mm3 and 1201.13 ± 42.15 mm3 vs 384.83 ± 4.76 mm3, P < 0.001; 21.87 ± 0.47 and 22.56 ± 0.71 vs 8.48 ± 0.53, P < 0.001, respectively). The expression of COX-2 protein was significantly lower in the MME-transfected group than that in the controls by Western blot analysis (2 ......
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