siRNA沉默STAT3基因对人肝癌细胞的抑制及对相关生长调控基因的调节
STAT3基因;肝癌;RNA干涉;pSilencer3.0-H1-siRNA-STAT3重组质粒;细胞凋亡,赵李静,朴云峰,丁百静,蒋政,通讯作者:,SilencingofSTAT3expressionbysiRNAsup
 |
| 第5页 |
 |
| 第1页 |
参见附件(716KB，7页)。
赵李静, 朴云峰, 丁百静, 吉林大学第一医院消化科 吉林省长春市 130021
蒋政, 锦州市第二医院骨科 辽宁省锦州市 121003
李静, 辽宁医学院附属第一医院消化科副教授, 硕士研究生导师, 主要从事消化系肿瘤的基因诊断与基因治疗的研究.
通讯作者: 李静, 130021, 吉林省长春市, 吉林大学第一医院消化科. jz7203@yahoo.com.cn
电话: 0431-84995918
收稿日期: 2007-03-31 接受日期: 2007-04-21
Silencing of STAT3 expression by siRNA suppresses the growth of human hepatocellular carcinoma cells and regulates genes related to growth control
Jing Li, Yun-Feng Piao, Zheng Jiang, Bai-Jing Ding
Jing Li, Yun-Feng Piao, Bai-Jing Ding, Department of Gastroenterology, the First Hospital of Jilin University, Changchun 130021, Jilin Province, China
Zheng Jiang, Department of Orthopaedics, the Second Hospital of Jinzhou City, Jinzhou 121003, Liaoning Province, China
Correspondence to: Jing Li, Department of Gastroenterology, the First Hospital of Jilin University, Changchun 130021, Jilin Province, China. jz7203@yahoo.com.cn
Received: 2007-03-31 Accepted: 2007-04-21
Abstract
AIM: To explore the effect of silencing STAT3 expression by siRNA on the growth of human hepatocellular carcinoma cells and the regulation of genes related to growth control.
METHODS: We reconstructed the recombinant plasmid of pSilencer 3.0-H1-STAT3-siRNA-GFP, which was then transfected into SMMC 7721 cells. MTT assay was applied to investigate the proliferation of transfected SMMC 7721 cells, and the protein expression level of STAT3 was determined by Western blotting and immunohistochemical staining. The transcription of the STAT3 gene was detected by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR), mRNA expression for growth-control-related genes such as survivin, c-myc, VEGF, p53 and caspase3 was detected in transfected cells at the same time. Flow cytometry (FCM) and AO/EB double staining were used to observe apoptosis in transfected cells.
RESULTS: MTT assay demonstrated that cell growth was inhibited in transfected cells, and the 48-hour and 72-hour inhibition rates of the test group were 59 ......
您现在查看是摘要介绍页，详见PDF附件(716KB，7页)。