多巴脱羧酶小干扰RNA体外对胃癌细胞侵袭转移能力的影响
多巴脱羧酶;小干扰RNA;胃癌;侵袭;转移;逆转录酶链式反应;免疫印迹,赵丹懿,徐惠绵,尚超,通讯作者:,EffectofdopadecarboxylasespecificsmallinterferingRNAoninvasionandme
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赵丹懿, 徐惠绵, 中国医科大学附属第一医院肿瘤外科 辽宁省沈阳市 110001
尚超, 中国医科大学神经生物学教研室 辽宁省沈阳市 110001
赵丹懿, 2004年中国医科大学肿瘤学博士生, 主要从事胃癌侵袭转移相关机制研究.
通讯作者: 徐惠绵, 110001, 辽宁省沈阳市, 中国医科大学附属第一医院肿瘤外科. xuhuimian@126.com
电话: 024-83283555
收稿日期: 2007-01-04 接受日期: 2007-05-16
Effect of dopa decarboxylase specific small interfering RNA on invasion and metastasis of gastric cancer cell
Dan-Yi Zhao, Hui-Mian Xu, Chao Shang
Dan-Yi Zhao, Hui-Mian Xu, Department of Surgical Oncology, First Affiliated Hospital to China Medical University, Shenyang 110001, Liaoning Province, China
Chao Shang, Department of Neurobiology, China Medical University, Shenyang 110001, Liaoning Province, China
Correspondence to: Hui-Mian Xu, Department of Surgical Oncology, First Affiliated Hospital to China Medical University, Shenyang 110001, Liaoning Province, China. xuhuimian@126.com
Received: 2007-01-04 Accepted: 2007-05-16
Abstract
AIM: To explore the effect of dopa decarboxylase (DDC) on the invasion and metastasis of gastric cancer cell.
METHODS: DDC-specific small interfering RNA was constructed in vitro and then transfected into human gastric cancer cell line BGC823. The mRNA and protein expression of DDC were examined by reverse transcriptase polymerase chain reaction (RT-PCR) and Western blotting methods, respectively. The invasive and metastatic ability of cancer cell were assessed by invasion assay in vitro.
RESULTS: After siRNA transfecting into human gastric cancer cell line BGC823, the expression of DDC mRNA and protein was significantly inhibited by RT-PCR and Western blot methods (0.27 ± 0.09 vs 0.89 ± 0.14; 0.39 ± 0.12 vs 1.26 ± 0.19, both P < 0.05); there were no significant changes in untransduced group and negative control group. The invasion assay demonstrated that the number of gastric cancer cell in transfection group was significantly lower than that in untransduced group and negative control group (6 ......
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