TFPI-2基因体外诱导胰腺癌Panc-1细胞的凋亡
胰腺肿瘤;细胞凋亡;组织因子途径抑制物2;免疫印迹;逆转录-聚合酶链反应,汤志刚,孙振阳,胡何节,许戈良,陈炯,李建生,汤志刚,通讯作者:,ApoptosisofPanc-1pancreaticcancercellsinducedbytheT
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汤志刚, 孙振阳, 胡何节, 许戈良, 陈炯, 李建生, 安徽医科大学附属安徽省立医院普通外科 安徽省合肥市 230001
汤志刚, 2004年华中科技大学同济医学院外科学博士, 副主任医师, 主要从事肝胆胰基础及临床研究.
安徽省自然科学基金资助项目, No. 050430702
通讯作者: 汤志刚, 230001, 安徽省合肥市, 安徽医科大学附属安徽省立医院普通外科. tzg7031@163.com
电话: 0551-2283280
收稿日期: 2007-04-13 接受日期: 2007-05-11
Apoptosis of Panc-1 pancreatic cancer cells induced by the TFPI-2 gene in vitro
Zhi-Gang Tang, Zhen-Yang Sun, He-Jie Hu, Ge-Liang Xu, Jiong Chen, Jian-Sheng Li
Zhi-Gang Tang, Zhen-Yang Sun, He-Jie Hu, Ge-Liang Xu, Jiong Chen, Jian-Sheng Li, Department of General Surgery, The Affiliated Anhui Province Hospital of Anhui Medical University, Hefei 230001, Anhui Province, China
Supported by: Anhui Province Natural Science Foundation, No. 050430702
Correspondence to: Zhi-Gang Tang, Department of General Surgery, The Affiliated Anhui Province Hospital of Anhui Medical University, Hefei 230001, Anhui Province,China. tzg7031@163.com
Received: 2007-04-13 Accepted: 2007-05-11
Abstract
AIM: To study the effect of the TFPI-2 gene on apoptosis of human pancreatic cancer cells.
METHODS: Recombinant pEGFP-C1-TFPI-2 was transfected into Panc-1 cells using liposomes. Positive cells were selected by G418. Expression of the TFPI-2 gene was determined by Reverse transcription-polymerase chain reaction(RT-PCR) and Western blotting. Cell growth and apoptosis were also studied.
RESULTS: Expression of TFPI-2 mRNA and protein was detected in Panc-1 cells transfected with pEGFP-C1-TFPI-2. Comparing Panc-1 cells transfected with vector and untransfected cells, the growth rate was lower and the apoptotic index of G418-resistant Panc-1 cells with the expression of TFPI-2 protein was faster(n = 9, P = 0.02).
CONCLUSION: The TFPI-2 gene can inhibit the growth of Panc-1 cells and cause cells to enter apoptosis.
Key Words: Pancreatic neoplasms; Apoptosis; TFPI-2; Western blotting; Reverse transcription-polymerase chain reaction ......
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