半枝莲黄酮类化合物对体外肿瘤血管生成的影响
半枝莲;黄酮类化合物;血管生成;人脐静脉内皮细胞;人宫颈癌Hela细胞;血管内皮细胞生长因子;一氧化氮;酶联免疫吸附测定法,徐敏,卜平,李瑶瑶,徐敏,通讯作者:,EffectsofanextractfromScutellariabarbataeD.
 |
| 第1页 |
参见附件(1000KB，5页)。
徐敏,卜平, 李瑶瑶, 扬州大学医学院中医系 江苏省扬州市 225001
徐敏, 2003年扬州大学医学院本科毕业, 2006年扬州大学医学院硕士研究生毕业, 医师, 主要从事消化系统肿瘤的研究.
江苏省自然科学基金资助项目, No. BK2001149
通讯作者: 卜平, 225001, 江苏省扬州市淮海路11号, 扬州大学医学院中医系. boping@yzu.edu.cn
电话: 0514-7978801
收稿日期: 2007-04-08 接受日期: 2007-04-28
Effects of an extract from Scutellaria barbatae D.DON on tumor angiogenesis in vitro
Min Xu, Ping Bu, Yao-Yao Li
Min Xu, Ping Bu, Yao-Yao Li,Department of Traditional Chinese Medicine, Medical College of Yangzhou University, Yangzhou 225001, Jiangsu Province, China
Supported by:Natural Science Foundation of Jiangsu Province, No. BK2001149
Correspondence to:Ping Bu, Department of Traditional Chinese Medicine, Medical College of Yangzhou University, Yangzhou 225001, 11 Huaihai Road, Jiangsu Province, China. boping@yzu.edu.cn
Received:2007-04-08 Accepted:2007-04-28
Abstract
AIM: To explore the effects of the extract flavonoid A06 from Scutellaria barbatae D.DON on tumor angiogenesis in vitro.
METHODS: Human umbilical venous endothelial cells (HUVECs) were treated with different concentrations of the flavonoid A06 (25, 50 and 100 mg/L). The effect of flavonoid A06 on the formation and migration of tubules in cultured HUVECs was evaluated by tube formation assay and Transwell chambers. The effect of flavonoid A06 on protein expression of vascular endothelial growth factor (VEGF) and nitric oxide (NO) in human cervical carcinoma Hela cells was detected by enzyme-linked immunosorbent assay and the nitrate reductase method.
RESULTS: Flavonoid A06 extract from Scutellaria barbatae D.DON at two concentrations (50 and 100 mg/L) effectively inhibited migration (37.7% ± 10.7%, 13.7% ± 6.0% vs 68.0% ± 8.2%, both P < 0.01) and tubule formation in HUVECs (9.7% ± 4.5%, 1.8% ± 1.2% vs 30.2% ± 5.4%, both P < 0.05) after 24 h. Flavonoid A06 also decreased the protein expression of VEGF and NO in Hela cells after 24 h (VEGF, 135 ......
您现在查看是摘要介绍页，详见PDF附件(1000KB，5页)。