白细胞介素6对豚鼠离体近端结肠平滑肌收缩的影响
白细胞介素6;河豚毒素;结肠平滑肌;肠神经;Cajal间质细胞,吕晓光,余保平,徐龙,吴志轩,吕晓光,通讯作者:,Effectsofinterleukin-6onthecontractionofcolonicsmoothmuscle,Xia
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吕晓光, 余保平, 徐龙, 吴志轩, 武汉大学人民医院消化内科 湖北省武汉市 430060
吕晓光, 2005年武汉大学人民医院消化内科硕士, 主要从事胃肠动力学研究
通讯作者: 余保平, 430060, 湖北省武汉市, 武汉大学人民医院消化内科. yubp62@163.com
电话: 027-68759391
收稿日期: 2007-04-04 接受日期: 2007-04-28
Effects of interleukin-6 on the contraction of colonic smooth muscle
Xiao-Guang Lv, Bao-Ping Yu, Long Xu, Zhi-Xuan Wu
Xiao-Guang Lv, Bao-Ping Yu, Long Xu, Zhi-Xuan Wu,Department of Gastroenterology, Renmin Hospital, Wuhan University, Wuhan 430060, Hubei Province, China
Correspondence to:Pro. Bao-Ping Yu, Department of Gastroenterology, Renmin hospital, Wuhan University, Wuhan 430060, Hubei Province, China. yubp62@163.com
Received:2007-04-04 Accepted: 2007-04-28
Abstract
AIM: To investigate the effects and mechanism of interleukin-6 on the contraction of colonic smooth muscle.
METHODS: Smooth muscle segments with mucosa were removed from the proximal colon of guinea pigs, and were divided into three groups: group A [interleukin-6 (IL-6) was added to Tyrode’s solution], group B (tetrodotoxin and IL-6 added), and group C (interstitial cells of Cajal were specially destroyed by methylene blue incubation and intensive illumination).
RESULTS: The amplitude and frequency of contractions were upregulated by IL-6 in a dose-independent manner. The frequency and amplitude of contractions were decreased when the enteric nerve was blocked by tetrodotoxin (0.206 ± 0.027 g vs 0.300 ± 0.039 g; 9.770 ± 1.711 s vs 8.483 ± 1.113 s; P < 0.01, P < 0.05). Addition of IL-6 (80 µg/L) after blocking with tetrodotoxin increased amplitude and frequency of smooth muscle contraction (P < 0.01, P < 0.05). The contraction of smooth muscle was nearly abolished when interstitial cells of cajal were destroyed by methylene blue incubation and intensive illumination (P < 0.01). Although IL-6 was administered, there was no significant difference in the amplitude and frequency of the contraction of smooth muscle segments when interstitial cells of cajal were destroyed by methylene blue and intensive illumination ......
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