抗突变中药对错配修复基因缺失结直肠癌细胞的抑制作用
中药;抗突变作用;结肠癌;错配修复;MTT法;多重荧光聚合酶链式反应;体外实验,刘秀芳,金黑鹰,丁义江,丁曙晴,刘飞,倪敏,王静,陆茵,李璘,刘秀芳,通讯作者:,InhibitoryeffectofChineseherbswithant
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刘秀芳, 金黑鹰, 丁义江, 丁曙晴, 刘飞, 倪敏, 王静, 南京中医药大学第三附属医院, 全国肛肠医疗中心, 江苏省南京市 210001
陆茵, 李璘, 南京中医药大学海洋药物研究所 江苏省南京市 210029
刘秀芳, 南京中医药大学研究生, 主要从事肿瘤遗传学方面的研究.
国家自然科学基金资助课题, No. 30572447
南京市医学发展重点项目资助课题, No. ZKX05028
通讯作者: 金黑鹰, 210001, 江苏省南京市金陵路1号, 南京中医药大学第三附属医院, 全国肛肠医疗中心.
jinheiying@yahoo.com.cn
电话: 025-86626137-6217 传真: 025-86627364
收稿日期: 2007-03-08 接受日期: 2007-04-21
Inhibitory effect of Chinese herbs with anti-mutation activity on a mismatch-repair-gene-deficient colorectal cancer cell line
Xiu-Fang Liu, Hei-Ying Jing, Yi-Jiang Ding, Yin Lu, Lin Li, Shu-Qing Ding, Fei Liu, Min Ni, Jing Wang
Xiu-Fang Liu, Hei-Ying Jing, Yi-Jiang Ding, Shu-Qing Ding, Fei Liu, Min Ni, Jing Wang,National Center of Colorectal Surgery, the Third Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Nanjing 210001, Jiangsu Province, China
Yin Lu, Lin Li,the Ocean Drug Institute of Nanjing University of Traditional Chinese Medicine, Nanjing 210029, Jiangsu Province, China
Supported by:National Natural Science Foundation of China, No. 30572447 and the Key Project of Medicine Development of Nanjing City, No. ZKX05028
Correspondence to:Hei-Ying Jing, National Center of Colorectal Surgery, the Third Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, No. 1 Jingling Road, Nanjing 210001, Jiangsu Province, China. jinheiying@yahoo.com.cn
Received:2007-03-08 Accepted:2007-04-21
Abstract
AIM: To study the inhibitory effect of Chinese herbs with anti-mutation activity on the mismatch-repair-gene-deficient colorectal cancer cell line.
METHODS: Fourteen Chinese herbs were used to treat the hMSH2-deficient colorectal cancer Lovo cell line and mismatch-repair-gene-deficient colorectal SW480 cell line, and growth rate was evaluated by the MTT method. The microsatellite instability change was examined by multiplex fluorescent polymerase chain reaction (PCR) ......
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