胃癌细胞SGC7901中STAT1, NF-κB p65和caspase 8的关系
胃癌;SGC7901细胞;信号转导子与转录活化因子1;核因子κBp65;caspase8;逆转录酶链式反应,胃癌细胞SGC7901中STAT1,NF-κBp65和caspase8的关系,向春香,邓昊,罗海莲,刘丽江,向春香,通讯作者:,Relationshipofsignaltransduc
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向春香, 邓昊, 罗海莲, 刘丽江, 江汉大学肿瘤研究所 湖北省武汉市 430056
向春香, 2005级武汉大学医学院病理学与病理生理学系硕士生, 现在江汉大学肿瘤研究所工作.
武汉市科技攻关计划引导项目, No. 20025007170
武汉市青年晨光计划项目, No. 20045006071-7
通讯作者: 刘丽江, 430056, 湖北省武汉市, 江汉大学肿瘤研究所. liulijiang@163.com
电话: 027-84226503
收稿日期: 2007-04-05 接受日期: 2007-04-28
Relationship of signal transduction and activators of transcription 1, nuclear factor κB p65 and caspase 8 in gastric cancer cell line SGC7901
Chun-Xiang Xiang, Hao Deng, Hai-Lian Luo, Li-Jiang Liu
Chun-Xiang Xiang, Hao Deng, Hai-Lian Luo, Li-Jiang Liu,Tumor Institute of Jianghan University, Wuhan 430056, Hubei Province, China
Supported by:The Key Item from Science and Technology Program of Wuhan City, No. 20025007170 and the Chenguang Project for Youth of Wuhan City, No. 20045006071-7
Correspondence to:Pro. Li-Jiang Liu, Tumor Institute of Jianghan University, Wuhan 430056, Hubei Province, China. liulijiang@163.com
Received:2007-04-05 Accepted:2007-04-28
Abstract
AIM: To discuss the relationship between signal transducer and activator of transcription 1 (STAT1), nuclear factor-κB (NF-κB) p65 and apoptotic factor casapase 8, to elucidate the possible mechanism of apoptosis in the human gastric cancer cell line SGC7901.
METHODS: SGC7901 cells were treated with IFN-g and STAT1 antisense oligonucleotides (ASONs). The mRNA expression for STAT1, NF-κB p65 and caspase 8 was examined by reverse transcription polymerase chain reaction (RT-PCR).
RESULTS: When SGC7901 cells were treated with IFN-g 1000 U, at 0.5, 3 and 24 h, the mRNA levels for STAT1 and NF-κB p65 expression were up-regulated; there was also a positive correlation between STAT1 and NF-κB p65 expression (r = 0.403, P = 0.009). With STAT1 ASONs, STAT1 expression was down-regulated, while NF-κB p65 expression was up-regulated; in the mRNA levels, there was a negative correlation between STAT1 and NF-κB p65 (r = -0 ......
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