大鼠酒精性肝病细胞凋亡与细胞色素P4502E1和氧化应激的关系
酒精性肝病;细胞凋亡;Caspase-3;细胞色素P4502E1;氧化应激;原位末端标记法;免疫组化法;聚合酶链式反应,金武丕,权修权,崔香丹,朴海今,孟繁平,金武丕,通讯作者:,Relationshipbetweenhep
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金武丕, 权修权, 崔香丹, 朴海今, 延边大学医院 吉林省延吉市 133000
孟繁平, 延边大学医学院 吉林省延吉市 133000
金武丕, 博士在读, 主任医师, 主要从事酒精性肝病的研究.
通讯作者: 金武丕, 133000, 吉林省延吉市, 延边大学医院急诊科. wpjin8933@163.com
电话: 0433-2660120
收稿日期: 2007-03-19 接受日期: 2007-04-13
Relationship between hepatocyte apoptosis, cytochome P4502E1 and oxidative stress in alcoholic liver disease in rats
Wu-Pi Jin, Xiu-Quan Quan, Fan-Ping Meng, Xiang-Dan Cui, Hai-Jin Piao
Wu-Pi Jin, Xiu-Quan Quan, Xiang-Dan Cui, Hai-Jin Piao,Affiliated Hospital of Yanbian University, Yanji 133000, Jilin Province, China
Fan-Ping Meng,Medical College of Yanbian University, Yanji 133000, Jilin Province, China
Correspondence to:Wu-Pi Jin, Department of Emergency, Hospital of Yanbian University, Yanji 133000, Jilin Province, China. wpjin8933@163.com
Received:2007-03-19 Accepted:2007-04-13
Abstract
AIM: To observe the pathological changes and investigate the relationship between hepatocyte apoptosis, cytochrome P4502E1 (CYP2E1) and oxygen free radicals in alcoholic liver disease (ALD) in rats.
METHODS: Ethanol (400 mL/L) was administered at 8 g/kg body weight by gavage twice daily for 8 weeks in the ALD model group, and the control group received isovolumic saline by gavage. By the end of the eighth week, the serum activity of alanine aminotransferase and aspartate aminotransferase were detected by automatic biochemistry analyzer. The pathological changes in the liver were observed by light microscopy of HE-stained sections; hepatocyte apoptosis was detected by the TdT-mediated d-UTP nick end labeling (TUNEL) method; and expression of caspase-3 in the liver was detected by immunohistochemistry. Expression of serum CYP2E1 was determined by polymerase chain reaction, and maleic dialdehyde (MDA) level and superoxide dismutase (SOD) activity in serum were detected by the thiobarbituric acid (TBA) method.
RESULTS: TUNEL-positive cells were located around the central vein, spotty and focal necrosis area in the ALD group, and Caspase-3 was observed around the central vein and areas of hepatocellular necrosis ......
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