CD4+CD25+调节性T细胞在慢性乙型肝炎患者免疫发病机制中的作用
调节性T细胞;乙型肝炎;慢性,CD4+CD25+调节性T细胞在慢性乙型肝炎患者免疫发病机制中的作用,张恒辉,郭芳,费然,马慧,丛旭,魏来,陈红松,王雪艳,张恒辉,通讯作者:,EffectsofregulatoryTcellsonanti-viralimmuneresponsesimpai
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张恒辉, 郭芳, 费然, 马慧, 丛旭, 魏来, 陈红松, 北京大学人民医院肝病研究所 北京市 100044
王雪艳, 北华大学医学院 吉林省吉林市 132000
张恒辉, 2005年北京大学内科学硕士研究生毕业, 研究实习员. 主要研究方向肝炎及肝癌的免疫治疗.
国家自然科学基金资助项目, No. 30640091
北京大学人民医院研究发展基金, No. RDB2006-01
通讯作者: 陈红松, 100044, 北京市西直门南大街11号, 北京大学人民医院肝病研究所. chen2999@sohu.com
电话: 010-88325726 传真: 010-68318386
收稿日期: 2007-04-09 接受日期: 2007-05-11
Effects of regulatory T cells on anti-viral immune responses impaired by chronic hepatitis B
Heng-Hui Zhang, Fang Guo, Ran Fei, Hui Ma, Xue-Yan Wang, Xu Cong, Lai Wei, Hong-Song Chen
Heng-Hui Zhang, Fang Guo, Ran Fei, Hui Ma, Xu Cong, Lai Wei, Hong-Song Chen,Hepatology Institute, Peking University, People’s Hospital, Beijing 100044, China
Xue-Yan Wang,Beihua University Medical College, Jilin 132000, Jilin Province, China
Supported by:National Natural Science Foundation of China, No. 30640091; Peking University, People’s Hospital Research and Development Foundation, No. RDB2006-01
Correspondence to:Hong-Song Chen, Vice Professor, Hepatology Institute, Peking University, People’s Hospital, No. 11 Xizhimen South Street, Beijing 100044, China. chen2999@sohu.com
Received:2007-04-09 Accepted:2007-05-11
Abstract
AIM: To evaluate the proportion of CD4+CD25+ regulatory T cells (Tregs) in the immunopathogenesis of patients with chronic hepatitis B (CHB), and to evaluate their potential application in CHB immunotherapy.
METHODS: Peripheral blood mononuclear cells (PBMCs) of 34 patients with CHB and 18 healthy donors were evaluated for the CD4+CD25+ Treg phenotype and the proportion of CD4+CD25+ Tregs as a percentage of the total number of CD4+ cells using flow cytometry with triple-color/fourth-color staining. CD4+CD25+ Tregs were isolated from CHB patients by magnetic beads. Then PBMCs of Treg-depleted and non-Treg-depleted CHB patients were stimulated with HBV core18–27 peptide ......
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