结肠特异性基因RELMβ的克隆及其真核表达载体构建
RELMβ基因;组织特异性;结肠;基因表达;免疫印迹;逆转录聚合酶链反应,郑丽端,杨秀萍,童强松,吕清,董继华,郑丽端,通讯作者:,Cloningofcolon-specificgeneresistin-likemoleculebetaan
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郑丽端, 杨秀萍, 华中科技大学同济医学院附属协和医院病理科 湖北省武汉市 430022
童强松, 吕清, 华中科技大学同济医学院附属协和医院外科 湖北省武汉市 430022
董继华, 华中科技大学同济医学院附属协和医院中心实验室 湖北省武汉市 430022
郑丽端, 医学博士, 主治医师, 主要从事消化系统疾病的分子病理研究.
国家自然科学基金资助项目, No. 30600278
通讯作者: 童强松, 430022, 湖北省武汉市, 华中科技大学同济医学院附属协和医院外科. qs_tong@hotmail.com
电话: 027-85726129
收稿日期: 2007-03-18 修回日期: 2007-07-24
Cloning of colon-specific gene resistin-like molecule beta and construction of its eukaryotic expression vector
Li-Duan Zheng, Qiang-Song Tong, Qing Lv, Xiu-Ping Yang, Ji-Hua Dong
Li-Duan Zheng, Xiu-Ping Yang, Department of Pathology, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China
Qiang-Song Tong, Qing Lv, Department of Surgery, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China
Ji-Hua Dong, Department of Central Laboratory, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China
Supported by: National Natural Science Foundation of China, No. 30600278
Correspondence to: Dr. Qiang-Song Tong, Department of Surgery, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China. qs_tong@hotmail.com
Received: 2007-03-18 Revised: 2007-07-24
Abstract
AIM: To clone mouse resistin-like molecule beta (RELMβ), a colon-specific gene, and construct its eukaryotic expression vector.
METHODS: RELMβ expression in diverse organs and tissues in mice was investigated by Western and Northern blotting. Total RNA was extracted from mouse colon tissues. Full-length cDNA of the RELMβ gene was amplified by reverse transcriptase-polymerase chain reaction (RT-PCR), inserted into T/A vector, validated by nucleic acid sequencing, and subcloned into the EcoRI restriction site of eukaryotic vector pcDNA3 ......
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