Bcl-2在肝细胞癌变过程中凋亡抑制作用的动态分析
肝癌;Bcl-2;免疫组化;酶联免疫吸附试验,宗蕾,姚登福,吴玮,邱历伟,吴信华,宗蕾,通讯作者:,DynamicanalysisofB-celllymphoma-2apoptoticinhibitoryeffectsduringthedeve
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宗蕾, 姚登福, 吴玮, 邱历伟, 吴信华, 南通大学附属医院医学实验中心 江苏省南通市 226001
宗蕾, 2006年南通大学肿瘤学硕士生, 主要从事肿瘤早期诊断及分子机制研究.
江苏省卫生厅项目, No. H200523
南通市社会发展科技计划, No. S5053
通讯作者: 姚登福, 226001, 江苏省南通大学附属医院医学实验中心. yaodf@ahnmc.com
电话: 0513-85052413
收稿日期: 2007-04-18 修回日期: 2007-07-17
Dynamic analysis of B-cell lymphoma-2 apoptotic inhibitory effects during the development of hepatocellular carcinoma
Lei Zong, Deng-Fu Yao, Wei Wu, Li-Wei Qiu, Xin-Hua Wu
Lei Zong, Deng-Fu Yao, Wei Wu, Li-Wei Qiu, Xin-Hua Wu, Research Center of Clinical Molecular Biology, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, China
Supported by: Jiangsu Provincial Bureau of Health, No. H200523; the Science and Technology Program of the Society Development in Nantong City, No. S5053
Correspondence to: Deng-Fu Yao, Research Center of Clinical Molecular Biology, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, China. yaodf@ahnmc.com
Received: 2007-04-18 Revised: 2007-07-17
Abstract
AIM: To investigate the dynamic expression and clinicopathological alterations of B-cell lymphoma-2 (Bcl-2) during the development of hepatocellular carcinoma (HCC).
METHODS: Hepatoma was induced by 2-fluorenylacetamide (2-FAA) in male Sprague-Dawley rats. Morphological changes in rat livers were observed by pathological examination (HE staining). Dynamic changes in Bcl-2 in rat livers were quantitatively analyzed by enzyme-linked immunosorbent assay. The expression and cellular distribution of liver Bcl-2 were simultaneously detected by immunohistochemistry.
RESULTS: Histological examination confirmed that rat hepatocytes changed from granule-like degeneration to atypical hyperplasia and HCC development after induction by 2-FAA. There were progressive increases in the level of hepatic Bcl-2 expression during the course. The levels of Bcl-2 in hepatoma tissues were significantly higher than those in normal and degenerative tissue (5 ......
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