胃癌组织RASSF1基因启动子区甲基化的意义
胃癌;RASSF1A;抑癌基因;甲基化特异性聚合酶链反应,李学彦,傅宝玉,通讯作者:,MethylationoftheRASSF1Agenepromoteringastriccarcinoma,Xue-YanLi,Bao-YuFu,Corresp
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李学彦, 傅宝玉, 中国医科大学附属一院消化科 辽宁省沈阳市 110003
通讯作者: 李学彦, 110016, 辽宁省沈阳市沈河区文化路83号, 沈阳军区总医院综合二科. xylhome001@sina.com
电话: 024-23051185
收稿日期: 2007-03-13 修回日期: 2007-07-15
Methylation of the RASSF1A gene promoter in gastric carcinoma
Xue-Yan Li, Bao-Yu Fu
Xue-Yan Li, Bao-Yu Fu, Department of Gastroenterology, Chinese Medical University, the First Affiliated Hospital, Shenyang110003, Liaoning Province, China
Correspondence to: Xue-Yan Li, Department of Oncology, Comprehensive Section 2, the PLA General Hospital of Shenyang of Chinese PLA, 83 WenHua Road, Shenhe District, Shenyang 110016, Liaoning Province, China. xylhome001@sina.com
Received: 2007-03-13 Revised: 2007-07-15
Abstract
AIM: To investigate the methylation status of the suppressor gene RASSF1A promoter in gastric carcinoma and benign gastric disease, and its clinicopathological significance.
METHODS: Methylation status of the RASSF1A promoter was detected by methylation specific-polymerase chain reaction (MS-PCR) in 32 patients with gastric carcinoma, adjacent non-carcinomatous gastric tissues, and 46 patients with benign gastric disease.
RESULTS: The frequency of RASSF1A promoter methylation in gastric carcinoma was 62.5% (20/32), and in adjacent non-carcinomatous gastric tissues, it was 3.1% (1/32), while in chronic shallow gastritis and chronic atrophic gastritis, it was 3.3% (1/30) and 37.5% (6/16), respectively. The rates of RASSF1A promoter methylation in gastric carcinoma and adjacent non-carcinomatous gastric tissues were significantly correlated (P < 0.01). Methylation of RASSF1A promoter in gastric carcinoma had no relationship with differentiation grade, lymph node metastasis and tumor size.
CONCLUSION: Methylation of RASSF1A promoter in gastric carcinoma may be an important molecular change and contribute to the development of gastric carcinoma.
Key Words: Gastric carcinoma; RASSF1A; Suppressor gene; Methylation specific-polymerase chain reaction ......
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