高迁移率族蛋白1对多形核白细胞黏附和游出肺毛细血管内皮细胞的影响及其机制
高迁移率族蛋白1;多形核白细胞;肺毛细血管内皮细胞;黏附;游出;CD11,CD18;流式细胞仪,杨智勇,陶京,周峰,熊炯炘,吴河水,王春友,通讯作者:,Effectandmechanismofhighmobilitygrou
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杨智勇, 陶京, 周峰, 熊炯炘, 吴河水, 王春友, 华中科技大学同济医学院附属协和医院胰腺外科 湖北省武汉市 430022
杨智勇, 1999年华中科技大学学士, 2002年华中科技大学硕士, 2005年华中科技大学博士, 主治医师. 主要从事胰腺疾病研究.
国家自然科学基金资助项目, No. 30600593
通讯作者: 杨智勇, 430022, 湖北省武汉市解放大道1277号, 华中科技大学同济医学院附属协和医院胰腺外科. dryzy@163.com
电话: 027-85351621
收稿日期: 2007-04-16 修回日期: 2007-07-11
Effect and mechanism of high mobility group box 1 protein on adhesion to pulmonary capillary endothelial cells and transendothelial migration of polymorphonuclear leucocytes
Zhi-Yong Yang, Jing Tao, Feng Zhou, Jiong-Xin Xiong, He-Shui Wu, Chun-You Wang
Zhi-Yong Yang, Jing Tao, Feng Zhou, Jiong-Xin Xiong, He-Shui Wu, Chun-You Wang, Department of Pancreatic Surgery, Xiehe Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China
Supported by: National Natural Science Foundation of China, No. 30600593
Correspondence to: Zhi-Yong Yang, Department of Pancreatic Surgery, Xiehe Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology, 1277 Jiefang Road, Wuhan 430022, Hubei Province, China. dryzy@163.com
Received: 2007-04-16 Revised: 2007-07-11
Abstract
AIM: To observe the effect of high mobility group box 1 protein (HMGB1) on adhesion to pulmonary capillary endothelial cells (PCECs) and transendothelial migration of polymorphonuclear leucocytes (PMNs), and to investigate the mechanism involved.
METHODS: PMNs and PCECs of rats were isolated and cultured. PCEC monolayers were cultured with different concentrations of rHMGB, 10, 10, 100, 1000 or 10 000 μg/L. The adhesion rate of PMNs was then detected. A migration model of a boyden chamber was used to evaluate chemotaxis of HMGB1 to PMNs. Transendothelial migration was also investigated. PMNs were cultured with 100 μg/L rHMGB1 and the expression of CD11a/CD18 and CD11b/CD18 was detected by flow cytometry ......
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