应用基因芯片技术筛选肝细胞癌相关基因
基因芯片;肝细胞癌;基因表达谱,张弘,黄介飞,黄晓平,黄东风,魏群,鲍柏军,华婷琰,通讯作者:,Genechipusedforscreeningdifferentiallyexpressedgenesinhepatocellularcarcinoma,Hong
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张弘, 黄介飞, 黄晓平, 黄东风, 魏群, 鲍柏军, 华婷琰, 南通大学附属医院消化内科 江苏省南通市 226001
张弘, 1981年南通医学院大专, 1991年南通医学院硕士, 副研究员, 主要从事消化肿瘤标志物和分子生物学研究.
江苏省卫生厅医学科研项目, No. H200330
通讯作者: 黄介飞, 226001, 江苏省南通市, 南通大学附属医院消化内科. jiefeihuang@163.com
电话: 0513-85806629 传真: 0513-85519820
收稿日期: 2007-04-05 修回日期: 2007-07-21
Genechip used for screening differentially expressed genes in hepatocellular carcinoma
Hong Zhang, Jie-Fei Huang, Xiao-Ping Huang, Dong-Feng Huang, Qun Wei, Bai-Jun Bao, Ting-Yan Hua
Hong Zhang, Jie-Fei Huang, Xiao-Ping Huang, Dong-Feng Huang, Qun Wei, Bai-Jun Bao, Ting-Yan Hua, Department of Gastroenterology, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, China
Supported by: Health Department of Jiangsu Province, No. H200330
Correspondence to: Dr. Jie-Fei Huang, Department of Gastroenterology, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, China. jiefeihuang@163.com
Received: 2007-04-05 Revised: 2007-07-21
Abstract
AIM: To ascertain the effectiveness of genechip for screening the same differentially expressed genes of hepatocellular carcinoma (HCC).
METHODS: Total RNAs were isolated from tissues of two cases of HCC and one normal liver by the TRIzol method, and then purified, reverse-transcribed to cDNA incorporating a biotin-labeling probe, and hybridized with genechip (covering 18 400 transcripts, representing 14 500 distinct genes). Picture signals of the fluorescence in the gene array were scanned and compared by computer analysis to the differential expression of the genes in the two tissues.
RESULTS: The same differentially expressed genes were 2756 (19.01%) and the same up regulated and downregulated genes were 1772 and 984, respectively, in two HCC and one normal liver tissues. These genes were initially classified according to function, and further analysis showed that they play an important role in the pathogenesis of HCC ......
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