刘臻, 许维雪, 张小薄, 刘宝林, 崔东旭, 中国医科大学盛京医院普外一科 辽宁省沈阳市 110004

    通讯作者: 刘臻, 110004, 辽宁省沈阳市三好街36号, 中国医科大学盛京医院普外一科. liuzhen1973@yahoo.com.cn

    电话: 024-81365657

    收稿日期: 2007-05-19 修回日期: 2007-09-02

    Expression of the CXC chemokine receptor 3 and its ligands in the livers of ischemia/reperfusion injured rats

    Zhen Liu, Wei-Xue Xu, Xiao-Bo Zhang, Bao-Lin Liu, Dong-Xu Cui

    Zhen Liu, Wei-Xue Xu, Xiao-Bo Zhang, Bao-Lin Liu, Dong-Xu Cui, the First Department of General Surgery, Shengjing Hospital of China Medical University, Shenyang 110004, Liaoning Province, China

    Correspondence to: Zhen Liu, the First Department of General Surgery, Shengjing Hospital of China Medical University, 36 Sanhao Street, Shengyang 110004, Liaoning Province, China. liuzhen1973@yahoo.com.cn

    Received: 2007-05-19 Revised: 2007-09-02

    Abstract

    AIM: To explore the expression of the CXC chemokine receptor 3 (CXCR3) and its ligands (IP-10, Mig) in the livers of ischemia/reperfusion (I/R) injured rats.

    METHODS: Thirty-two Wistar rats were randomly divided into four groups, with 8 rats in each group: sham operation (SO) and 6-, 12- and 24-hour I/R groups. The level of tumor necrosis factor (TNF)-α in liver tissue was measured by enzyme-linked immunosorbent assay (ELISA). The expression levels of CXCR3 and its ligands (IP-10, Mig) were assessed by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). The serum levels of alanine transaminase (ALT) and aspartate transaminase (AST) were also analyzed. ......