在Fas诱导Bel-7402细胞凋亡中p38MAPK调节Bcl-2的表达
p38MAPK;p-p38MAPK;Bcl-2;凋亡;免疫印迹;逆转录聚合酶链式反应,王玉,孙黎光,叶丽平,张莹,王玉,夏春辉,王玉,通讯作者:,p38mitogen-activatedproteinkinasemo
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王玉, 孙黎光, 叶丽平, 张莹, 中国医科大学基础医学院生物化学与分子生物学教研室 辽宁省沈阳市 110001
王玉, 夏春辉, 齐齐哈尔医学院 黑龙江省齐齐哈尔市 161042
王玉, 副教授, 2005届中国医科大学博士生, 主要从事肝癌细胞生物学研究.
辽宁省教育厅科研基金资助项目, No. 2004D173
黑龙江省普通高等学校骨干教师创新能力资助计划, No. 1054G070
通讯作者: 孙黎光, 110001, 辽宁省沈阳市, 中国医科大学生物化学与分子生物学教研室. ydslg@163.com
电话: 024-23256666-5297
收稿日期: 2007-06-13 修回日期: 2007-10-07
p38 mitogen-activated protein kinase modulates Bcl-2 expression during FAS-induced apoptosis in Bel-7402 cells
Yu Wang, Li-Guang Sun, Chun-Hui Xia, Li-Ping Ye, Ying Zhang
Yu Wang, Li-Guang Sun, Li-Ping Ye, Ying Zhang,Department of Biochemistry and Molecular Biology, China Medical University, Shenyang 110001, Liaoning Province, China
Yu Wang, Chun-Hui Xia, Qiqihar Medical College, Qiqihar 161042, Heilongjiang Province, China
Supported by: the Scientific Research Foundation of the Education Department in Liaoning Province, No. 10511129, the Plan for the Core Teacher′s Innovation Abilities of Common Colleges and Universities in Heilongjiang Province, No. 1054G070
Correspondence to: Li-Guang Sun, Department of Biochemistry and Molecular Biology, China Medical University, Shenyang 110001, Liaoning Province, China. ydslg@163.com
Received: 2007-06-13 Revised: 2007-10-07
Abstract
AIM: To investigate whether p38 mitogen-activated protein kinase (p38MAPK) is involved in Fas- and actinomycin D (AD)-induced apoptosis in Bel-7402 cells, and the relationship between p38MAPK and Bcl-2 expression.
METHODS: We measured the viability of Bel-7402 cells by MTT assay, p38MAPK, p-p38MAPK and Bcl-2 expression by Western blotting and reverse transcription polymerase chain reaction (RT-PCR), and the location of p-p38MAPK in Bel-7402 cells after Fas and AD treatment by immunofluorescene.
RESULTS: Bel-7402 cell viability was significantly inhibited by Fas (P < 0.01). p38MAPK and p-p38MAPK increased significantly with increasing Fas (P < 0 ......
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