高迁移率族蛋白B1在重症急性胰腺炎肠屏障损害中的作用
重症急性胰腺炎;肠黏膜屏障;二硫代氨基甲酸吡咯烷;高迁移率族蛋白B1;逆转录-聚合酶链式反应;免疫印迹,栾正刚,马晓春,张成,葛春林,郭仁宣,栾正刚,通讯作者:,Roleofhighmobilitygroupbox-1proteinin
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栾正刚, 马晓春, 中国医科大学附属第一医院重症医学科 辽宁省沈阳市 110001
张成, 葛春林, 郭仁宣, 中国医科大学附属第一医院普外二科 辽宁省沈阳市 110001.
栾正刚, 主治医师, 博士, 主要从事重症急性胰腺炎发病机制方面的研究.
通讯作者: 郭仁宣, 110001, 辽宁省沈阳市, 中国医科大学附属第一医院普外二科. zgl17698@sina.com
电话: 024-83283330
收稿日期: 2007-06-14 修回日期: 2007-10-08
Role of high mobility group box-1 protein in gut mucosal barrier dysfunction during severe acute pancreatitis in rats
Zheng-Gang Luan, Cheng Zhang, Chun-Lin Ge, Xiao-Chun Ma, Ren-Xuan Guo
Zheng-Gang Luan, Xiao-Chun Ma, Department of Intensive Care Unit, the First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China
Cheng Zhang, Chun-Lin Ge, Ren-Xuan Guo, Department of General Surgery, the First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China
Correspondence to: Dr. Ren-Xuan Guo, Department of General Surgery, the First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China. zgl17698@sina.com
Received: 2007-06-14 Revised: 2007-10-08
Abstract
AIM: To investigate the role of high mobility group box-1 protein (HMGB1) in gut mucosal barrier dysfunction during severe acute pancreatitis (SAP) in mice, and the mechanisms involved.
METHODS: A rat model of SAP was established by retrograde injection of 50 g/L sodium taurocholate into the choledochopancreatic duct. Fifty-six healthy male Wistar rats were divided randomly into three groups: control, SAP (3, 6, 12, 24 and 48 hours subgroups), and pyrrolidine dithiocarbamate (PDTC). Plasma lipopolysaccharide (LPS) and blood diamine oxidase (DAO) levels were determined. The expression of HMGB1 mRNA in the intestinal mucosa was detected by reverse-transcription polymerase chain reaction (RT-PCR), and the activity of HMGB1 was determined by Western blotting.
RESULTS: Compared with the normal control group, HMGB1 mRNA level markedly increased in intestinal mucosa 6 hours after SAP (0 ......
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