中药胃肠舒对胃肠平滑肌细胞ATP生成的影响
中药;胃肠舒;ATP;线粒体跨膜电位;Celltiter-GloTM法;Rhodaminefluorescence法,宋晓冬,张丽霞,牛新华,闫苗苗,王晓江,刘孟安,孙丰润,刘颖,宋晓冬,通讯作者:,EffectoftraditionalChin
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宋晓冬, 张丽霞, 牛新华, 闫苗苗, 王晓江, 滨州医学院基础学院 山东省滨州市 256603
刘孟安, 孙丰润, 刘颖, 滨州医学院临床学院 山东省滨州市 256603
宋晓冬, 复旦大学硕士, 副教授, 主要从事中药机制研究.
国家自然科学基金资助项目, No. 30672680
通讯作者: 宋晓冬, 256603, 山东省滨州市, 滨州医学院基础学院. songxd71@yahoo.com.cn
电话: 0535-6913151
收稿日期: 2007-05-29 修回日期: 2007-09-29
Effect of traditional Chinese medicine Weichangshu on ATP in gastrointestinal smooth muscle cells
Xiao-Dong Song, Meng-An Liu, Feng-Run Sun, Ying Liu, Li-Xia Zhang, Xin-Hua Niu, Miao-Miao Yan, Xiao-Jiang Wang
Xiao-Dong Song, Li-Xia Zhang, Xin-Hua Niu, Miao-Miao Yan, Xiao-Jiang Wang, the Basic School of Binzhou Medical College, Binzhou 256603, Shandong Province, China
Meng-An Liu, Feng-Run Sun, Ying Liu, Clinical School of Binzhou Medical College, Binzhou 256603, Shandong Province, China
Supported by:National Natural Science Foundation of China, No. 30672680
Correspondence to:Xiao-Dong Song, Department of Biology, Binzhou Medical College, Binzhou 256603, Shandong Province, China. songxd71@yahoo.com.cn
Received:2007-05-29 Revised:2007-09-29
Abstract
AIM: To study the effect of Weichangshu on the kinetic energy of gastrointestinal smooth muscle cells.
METHODS: Gastrointestinal smooth muscle cells were divided into five groups: control, cultured in normal medium; cisapride, cultured in normal medium with 0.51 g/L cisapride; Weichangshu Ⅰ, cultured in normal medium with 25 g/L Weichangshu; Weichangshu Ⅱ, cultured in normal medium with 50 g/L Weichangshu; and Weichangshu Ⅲ, cultured in normal medium with 75 g/L Weichangshu. Celltiter-Glo was used to detect ATP. The relative changes in mitochondrial potential were tested with the rhodamine fluorescence (R-123) technique ......
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