肝癌组织中14-3-3基因差异表达的意义
14-3-3基因;肝癌;肝硬化;基因芯片,刘娟,姚树坤,殷飞,通讯作者:,Significanceofdifferentiallyexpressedgene14-3-3inhepatocellularcarcinoma,JuanLiu,Shu-KunYao
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刘娟, 姚树坤, 殷飞, 河北医科大学第四医院消化内科 河北省石家庄市 050011
刘娟, 2007届河北医科大学博士研究生, 医师, 主要从事消化系统疾病的研究.
河北省卫生厅科研基金自助项目, No. 07122
通讯作者: 殷飞, 050011, 河北省石家庄市健康路12号, 河北医科大学第四医院消化内科. yinfei_4y@sina.com
电话: 0311-86033941-342 传真: 0311-86077634
收稿日期: 2007-06-06 修回日期: 2007-10-10
Significance of differentially expressed gene 14-3-3 in hepatocellular carcinoma
Juan Liu, Shu-Kun Yao, Fei Yin
Juan Liu, Shu-Kun Yao, Fei Yin, Department of Gastroenterology, the Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei Province, China
Supported by: the Scientific Research Foundation of the Healthy Department in Hebei Province, No. 07122
Correspondence to: Dr. Fei Yin, Department of Gastroenterology, the Fourth Hospital of Hebei Medical University, 12 Jiankang Road, Shijiazhuang 050011, Hebei Province, China. yinfei_4y@sina.com
Received: 2007-06-06 Revised: 2007-10-10
Abstract
AIM: To detect the expression of 14-3-3 gene family members in hepatocellular carcinoma (HCC) by GeneChip and determine its clinical significance.
METHODS: Total RNA was isolated from tissue of HCC, liver cirrhosis and normal liver, purified into mRNA by Oligotex, and reverse-transcribed to synthesize two kinds of fluorescently labeled cDNA probes (Cy3-dUTP, Cy5-dUTP). The targets were mixed together and hybridized by GeneChip. The expression of 14-3-3 gene family members among different liver tissues was detected based on signal-to-noise ratios by using GenePix Pro3.0 software, the outcome was verified by semi-quantitative RT-PCR, and the clinical significance of differentially expressed genes was explored.
RESULTS: 14-3-3 gene family members were expressed differently in HCC, 14-3-3γ was down-regulated and closely related to the integrity of the envelope of the tumor. 14-3-3η was up-regulated in HCC and had a close relationship to the clinical stage of HCC ......
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