小鼠心脏移植后穿孔素及颗粒酶B表达水平与急性排斥反应的关系
穿孔素;颗粒酶;移植;免疫印迹;免疫荧光方法,朱鹏,陈义发,李丹,陈孝平,通讯作者:,Relationshipbetweenacuterejectionafterhearttransplantationandproteinexpressi
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朱鹏, 陈义发, 李丹, 陈孝平, 华中科技大学同济医学院附属同济医院肝脏外科中心 湖北省武汉市 430030
朱鹏, 2005年华中科技大学同济医学院硕士研究生毕业, 博士生, 主要从事肝胆外科及器官移植方面的研究.
国家自然科学基金资助项目, No. 30571754
通讯作者: 陈孝平, 430030, 湖北省武汉市解放大道1095号, 武汉同济医院肝脏外科中心. chenxp@medmail.com.cn
电话: 027-83662851 传真: 027-83662851
收稿日期: 2007-05-14 修回日期: 2007-09-24
Relationship between acute rejection after heart transplantation and protein expression of perforin and granzyme B
Peng Zhu, Yi-Fa Chen, Dan Li, Xiao-Ping Chen
Peng Zhu, Yi-Fa Chen, Dan Li, Xiao-Ping Chen, Hepatic Surgery Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei province, China
Supported by: National Natural Science Foundation of China, No. 30571754
Correspondence to: Prof. Xiao-Ping Chen, Hepatic Surgery Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan 430030, Hubei Province, China. chenxp@medmail.com.cn
Received: 2007-05-14 Revised: 2007-09-24
Abstract
AIM: To investigate the relationship between acute rejection after heart transplantation and protein expression of perforin and granzyme B in organ recipients.
METHODS: All ventral heterotopic cardiac transplantation models were divided into three groups: rejection, treated, and isograft (20 recipients in each). Mean survival time (MST), histopathological changes, and protein expression of perforin and granzyme B in spleen lymphocytes were measured by Western blotting. Immunofluorescence techniques were used to determine the graft expression of perforin and granzyme B.
RESULTS: MST of heart allografts in the rejection and treated groups was 7.8 ± 0.77 d and 14.80 ± 1.01 d, respectively. Graft survival time in the isograft group was > 28 d, The differences between the three groups were statistically significant. The number of infiltrating cells in the rejection group, most of which were positive for perforin and granzyme B protein expression, was much higher than that in the other groups, as was the extent of the histopathological changes ......
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