HER-2基因靶向RNA干扰重组表达载体的构建及序列分析
HER-2基因;RNA干扰;重组表达载体;短发夹RNA,韩明阳,吴爱国,李鹏,纪术峰,郭爱林,刘铮,通讯作者:,CloningofrecombinantplasmidaffectingHER-2ingenetranslationbyRN
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韩明阳, 吴爱国, 李鹏, 纪术峰, 南方医科大学珠江医院普通外科 广东省广州市 510280
郭爱林, 广东省人民医院医学研究中心 广东省广州市 510080
刘铮, 南方医科大学病理生理实验室 广东省广州市 510515
韩明阳, 南方医科大学珠江医院普通外科硕士生, 主要从事大肠癌的RNA干扰研究.
广东省科技计划项目, No. 2005B31201011
通讯作者: 吴爱国, 510280, 广东省广州市工业大道中253号, 南方医科大学珠江医院普通外科. yang07641@126.com
收稿日期: 2007-06-15 修回日期: 2007-09-13
Cloning of recombinant plasmid affecting HER-2 in gene translation by RNA interference
Ming-Yang Han, Ai-Guo Wu, Ai-Lin Guo, Peng Li, Shu-Feng Ji, Zheng Liu
Ming-Yang Han, Ai-Guo Wu, Peng Li, Shu-Feng Ji, Department of Surgery, Zhujiang Hospital, Nanfang Medical University, Guangzhou 510280, Guangdong Province, China
Ai-Lin Guo, Medical Research Center, Guangdong Provincial People’s Hospital, Guangzhou 510080, Guangdong Province, China
Zheng Liu, Pathophysiology and Key Laboratory, Nanfang Medical University, Guangzhou 510515, Guangdong Province, China
Supported by: Science and Technique Program of Guangdong Province, No. 2005B31201011
Correspondence to: Ai-Guo Wu, Department of Surgery, Zhujiang Hospital, Nanfang Medical University, 253 Industrial Road, Guangzhou 510280, Guangdong Province, China. yang07641@126.com
Received: 2007-06-15 Revised: 2007-09-13
Abstract
AIM: To clone the recombinant plasmid affecting HER-2 gene translation by RNA interference, and to analyze the nucleic acid sequence of the recombinant plasmid for tumor gene therapy.
METHODS: Two DNA sequences containing a small hairpin structure were designed and synthesized. The complement form was obtained by annealing and cloning into vector pGPU6/GFP/Neo, and the recombinant plasmid was transformed into strain DH5α. The plasmid identified by restriction enzyme analysis was used for sequencing. Human colon cancer HT29 cells were transfected with plasmid vector, and then fluorescence photographs were taken and selected according to G418 ......
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