一种新的基因灭活工具——甲基化寡核苷酸技术
甲基化;甲基化寡核苷酸;基因灭活,翟荣林,王国斌,蔡开琳,通讯作者:,Applicationofmethylatedoligonucleotides:Anewstrategyforgeneinactivation,Rong-LinZhai,Guo-Bin
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翟荣林, 王国斌, 蔡开琳, 华中科技大学同济医学院附属协和医院普外科 湖北省武汉市 430022
国家自然科学基金资助课题, No. 30500488
通讯作者: 翟荣林, 430022, 湖北省武汉市, 华中科技大学同济医学院附属协和医院普外科. zhaironglin@yahoo.com.cn
电话: 027-85351661
收稿日期: 2007-08-30 修回日期: 2007-09-28
Application of methylated oligonucleotides: A new strategy for gene inactivation
Rong-Lin Zhai, Guo-Bin Wang, Kai-Lin Cai
Rong-Lin Zhai, Guo-Bin Wang, Kai-Lin Cai, Department of General Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China
Supported by: National Natural Science Foundation of China, No. 30500488
Correspondence to: Rong-Lin Zhai, Department of General Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China. zhaironglin@yahoo.com.cn
Received: 2007-08-30 Revised: 2007-09-28
Abstract
The study of gene functions, including activation and inactivation, has become a hotspot in molecular biology since entering the postgenomic phase. To achieve gene inactivation, there are four methods: gene knock out, antisense oligonuleotides, RNA interference and methylated oligonucleotides. Here, we emphasize the technology of methylated oligonucleotides via backgrounds, mechanism, method, current status and perspective.
Key Words: Methylation; Methylated oligonuleotides; Gene inactivation
Zhai RL, Wang GB, Cai KL. Application of methylated oligonucleotides: A new strategy for gene inactivation. Shijie Huaren Xiaohua Zazhi 2007; 15(31): 3332-3337
摘要
后基因组时代的分子生物学研究重点转向了基因功能的研究. 基因功能研究中涉及到对过度表达的基因进行抑制或者灭活. 本文简要回顾和评价了基因敲除、反义寡核苷酸技术和RNA干扰技术等常用的基因灭活工具, 着重对有可能成为第四代基因灭活工具的甲基化寡核苷酸技术, 从产生背景、作用机制、技术路线、应用现状及前景等方面进行了详细的论述, 以期引起注意和重视.
关键词: 甲基化; 甲基化寡核苷酸; 基因灭活
翟荣林, 王国斌, 蔡开琳. 一种新的基因灭活工具——甲基化寡核苷酸技术. 世界华人消化杂志 2007;15(31):3332-3337
0 引言
肿瘤和遗传性疾病往往涉及多个基因功能的改变 ......
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