环孢素A对顺铂诱导肝癌细胞凋亡的影响
肝癌;环孢素;顺铂;凋亡;噻唑蓝比色法;流式细胞仪,曾兆林,崔云甫,彭威,通讯作者:,EffectofcyclosporineAoncisplatin-inducedapoptosisofhepatocellularcarcinomacells,Zhao-Li
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曾兆林, 崔云甫, 彭威, 哈尔滨医科大学附属第二医院普外科 黑龙江省哈尔滨市 150086
曾兆林, 1989年哈尔滨医科大学学士, 2005年哈尔滨医科大学外科学博士, 副主任医师, 主要从事普通外科的临床工作.
黑龙江省自然科学基金资助项目, No. 0331
通讯作者: 曾兆林, 150086, 黑龙江省哈尔滨市南岗区学府路246号, 哈尔滨医科大学附属第二医院普外科. zengzhaolin@sina.com
电话: 0451-86605356
收稿日期: 2006-12-29 修回日期: 2007-10-14
Effect of cyclosporine A on cisplatin-induced apoptosis of hepatocellular carcinoma cells
Zhao-Lin Zeng, Yun-Fu Cui, Wei Peng
Zhao-Lin Zeng, Yun-Fu Cui, Wei Peng, Department of General Surgery of the Second Affiliated Hospital, Harbin Medical University, Harbin 150086, Heilongjiang Province, China
Supported by: the Natural Science Foundation of Heilongjiang Province, No. 0331
Correspondence to: Dr. Zhao-Lin Zeng, Department of General Surgery, the Second Affiliated Hospital of Harbin Medical University, 246 Xuefu Road, Harbin 150086, Heilongjiang Province, China. zengzhaolin@sina.com
Received: 2006-12-29 Revised: 2007-10-14
Abstract
AIM: To investigate the effect of cyclosporine A (CsA) on cisplatin (CDDP)-induced apoptosis of hepatocellular carcinoma (HCC) cells.
METHODS: SMMC 7721 HCC cells were cultured (re-cultured every 3 d) and divided into six groups: A (control), B (10 mg/L CsA), C (40 mg/L CsA), D (20 mg/L CDDP), E (10 mg/L CsA + 20 mg/L CDDP), and F (40 mg/L CsA + 20 mg/L CDDP). Morphological changes were observed by fluorescence microscopy, cell growth was assessed with the methyl thiazolyl tetrazolium (MTT) assay at 24 and 48 h, and apoptosis was assessed by flow cytometry.
RESULTS: The number of necrotic cells in groups E and F was more and the number of apoptotic HCC cells was significantly lower in groups E and F than in group D. The apoptotic rate was significantly lower in groups E (22.7% ± 2.4%) and F (22.8% ± 2.8%) than in group D (32.7% ± 2.8%, P = 0.008). The rate of apoptosis in groups A, B and C was the lowest, although there were no obvious differences between the groups ......
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